TISSUE INTERACTIONS AND SEMINAL VESICLE SECRETION

组织相互作用和精囊分泌

• 批准号: 3321053
• 负责人: GERALD R CUNHA
• 金额: $ 9.48万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3321053
• 关键词: androgens; autoradiography; cell cell interaction; cell differentiation; cell growth regulation; epithelium; gel electrophoresis; gene expression; genetic manipulation; hormone regulation /control mechanism; immunofluorescence technique; implant; mesenchyme; radiotracer; secretion; seminal vesicles; testicular feminization; tissue /cell culture

The factors responsible for the development of an immune response to spermatozoa in some individuals but not in others, have not been eludicated. Recent data have led to the hypothesis, to be tested in the present application, that suppressor T lymphocytes present in semen and/or the ability of seminal fluid to activate suppressor cells aid in minimizing an immune response to spermatozoa in males and females. Sera and peripheral blood mononuclear cells (PBMN) will be obtained from 100 male and female partners with primary infertility and 35 male and female partners of fertile marriages. Semen will be obtained from all men. All sera and semen will be tested for sperm and seminal fluid-reactive antibodies. The numbers of suppressor and helper T lymphocytes in the ejaculates will be quantitated with monoclonal antibodies. The ability of seminal lymphocytes to block in vitro proliferation and immunoglobulin production by the semen donor's PBMN, by those of his spouse or of control PBMN, will be determined. Similarly, the induction of suppressor cell activity in PBMN of husband and wife following incubation with husband or control seminal fluid will be assessed. To ascertain the origin of the seminal fluid component which induces suppressor cell activity, pre- and post-vasovasostomy seminal fluids from 30 men, as well as epididymal fluid obtained at the time of surgery, will be incubated with PBMN and tested for their effect on lymphocyte proliferation and immunoglobulin production. To determine the cell type(s) required for seminal fluid activation of suppressor cells, macrophages, T lymphocytes and B lymphocytes will be isolated, incubated alone or in various combinations with seminal fluid and tested for immunosuppressive properties.

导致免疫反应发展的因素 精子在一些个体中没有，但在另一些个体中，还没有 难以捉摸。最近的数据导致了这一假说，将在 目前应用是，存在于精液和/或 精液激活抑制细胞的能力有助于最小化 男性和女性对精子的免疫反应。血清和 将从100名男性中获取外周血单个核细胞(PBMN) 患有原发不孕症的女性伴侣和35名男性和女性 生育婚姻的伴侣。精液将从所有男人身上获得。全 将对血清和精液进行精子和精液反应测试 抗体。外周血中抑制性T淋巴细胞和辅助性T淋巴细胞的数量 射出的精液将用单抗进行定量。的能力 精液淋巴细胞体外阻断增殖和免疫球蛋白的作用 精液捐献者的PBMN、其配偶的PBMN或对照的PBMN PBMN，将被确定。同样，抑制细胞的诱导 与丈夫或妻子孵育后夫妻外周血单个核细胞的活性 将对对照精液进行评估。为了确定这一现象的起源 诱导抑制细胞活动的精液成分，前和 30例男性输精管吻合术后精液及附睾液 在手术时获得的，将与PBMN孵育并检测 它们对淋巴细胞增殖和免疫球蛋白产生的影响。至 确定激活精液所需的细胞类型(S) 抑制细胞、巨噬细胞、T淋巴细胞和B淋巴细胞 分离，单独孵化或与精液和精液各种组合培养 进行了免疫抑制性能测试。