HINDLIMB MUSCLE ORGANOGENESIS AND NEURAL INTEGRATION

后肢肌肉器官发生和神经整合

• 批准号: 3319068
• 负责人: Marcia R. Ontell
• 金额: $ 7.6万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-07-01 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3319068
• 关键词: early embryonic stage; electron microscopy; embryo /fetus; growth /development; histochemistry /cytochemistry; histogenesis; immunochemistry; infant animal; innervation; isozymes; leg; mammalian embryology; morphology; motor neurons; myogenesis; neuromuscular function

Developmentally-caused limb defects constitute a major debilitating disorder. In an attempt to evaluate the extent of variability from the normal pattern of limb development, it is necessary that the various systems contributing to normal limb development be systematically and thoroughly evaluated. It is proposed to study, in depth, the development, maturation and neural integration of hindlimb musculature, with emphasis on the following objectives: 1. Determination of the pattern of organogenesis of the fetal mouse soleus muscle (a predominantly slow twitch muscle). This pattern of development will be compared and contrasted with the pattern of development of the extensor digitorum longus muscle (a fast twitch muscle). 2. Investigation of the complex interrelationship between the developing muscle organ and the nervous system during the fetal and early neonatal stages, including: a. Establishment of the time course and pattern of myoneural integration. b. Investigation of mechanisms which may be involved in the establishment of polyneuronal innervation. c. Correlation of developmental events occurring in fetal muscle with maturational changes in the spinal cord (i.e. programmed motoneuron cell death). 3. Investigation of the time course of myosin transition in developing myofibers of future fast and slow hindlimb muscles, with emphasis of isozyme transitions in single motor units during the period of polyneuronal elimination. In vivo studies of developing muscles of the hindlimb of the 129 ReJ mouse will be carried out using spaced serial, section electron microscopic techniques, computer assisted morphometric analyses, retrograde transport of horseradish peroxidase, physiological and immunocytochemical techniques.

由发育引起的肢体缺陷是造成身体虚弱的主要原因 无序。为了评估可变性的程度 肢体发育的正常模式，需要不同的 促进正常肢体发育的系统应系统地和 彻底评估。 建议深入研究神经的发育、成熟和神经功能。 后肢肌肉的整合，重点是以下几点 目标： 1.胎鼠比目鱼肌器官发生模式的测定 肌肉(主要是缓慢的抽搐肌肉)。这种发展模式 将与中国的发展模式进行比较和对比。 指长伸肌(一种快速抽动的肌肉)。 2.发展与发展之间复杂相互关系的考察 胎儿和早期新生儿的肌肉器官和神经系统 阶段，包括： 建立肌神经整合的时间进程和模式。 B.调查可能参与设立的机制 多神经元神经支配。 C.胎儿肌肉中发生的发育事件与 脊髓的成熟性变化(即程序化运动神经元细胞 死亡)。 3.肌球蛋白在发育过程中转化的时程研究 未来快速和缓慢后肢肌肉的肌纤维，重点是 多神经元发育过程中单个运动单位的同工酶变化 淘汰赛。 129只REJ小鼠后肢肌肉发育的活体研究 将使用间隔的系列、切片电子显微镜进行 技术、计算机辅助形态分析、逆行运输 辣根过氧化物酶、生理和免疫细胞化学技术。