HUMAN YEAST ARTIFICIAL CHROMOSOME LIBRARY DATABASE

人类酵母人工染色体文库数据库

• 批准号: 3333264
• 负责人: Mark E Frisse
• 金额: $ 24.75万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-01 至 1994-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3333264
• 关键词: DNA; artificial chromosomes; biomedical resource; clone cells; computer system design /evaluation; fungal genetics; genetic library; human genetic material tag; human tissue; information retrieval; information systems; molecular cloning; molecular genetics

The Washington University Center for Genetics in Medicine has an extensive yeast artificial chromosome (YAC) library, consisting of over 50,000 clones with large inserts of human genomic DNA. These clones are used by investigators both inside and outside the University to study the structure and function of genes contributing to a variety of generic disorders. The continued growth in the size and the use of the clone library threatens our ability to manage the library and to respond to new research opportunities. We propose to develop a comprehensive information management environment that will help us to exploit the full potential of the YAC library. In constructing this information management environment, we will address a number of key research issues of direct relevance to other biomedical research laboratories facing similar information management crises: 1. We will develop a detailed representation of YAC library screening experiments to be used as a conceptual model for a comprehensive YAC clone information system which represents the entire range of laboratory activities. 2. We will design a conceptual data model and a formal database schema for a YAC library information system so that technological and methodological changes in YAC cloning methods can readily be incorporated. This is a significant research issue in information systems development in that traditional data models assume that the "world" represented in the database is relatively static. Because of the rapid improvements in genetics research methodology, our data models must be flexible enough to incorporate new developments. 3. We will implement a comprehensive database management system, derived from the formal data model, to support ongoing YAC research activities. This system will identify clones and primer pairs by "sequence-tagged sites" (STS), and it will facilitate collaborative research arrangements by tracking clones which have been sent to or received from outside investigators and by recording YAC structural information provided by these investigators. 4. We will develop a robust data acquisition environment so that laboratory personnel can enter experimental results directly into the information system. The goal is to eliminate the need for error-prone transcriptions of experimental results. A research team composed of geneticists and medical computer scientists will achieve these goals by creating a state-of-art, comprehensive YAC information management environment that is accessible, useful, reliable, and responsive to technological change, and that may ultimately serve as a central component of a more comprehensive "molecular genetics workstation."

华盛顿大学医学遗传学中心拥有广泛的 酵母人工染色体 (YAC) 文库，包含 50,000 多个克隆 带有大量人类基因组 DNA 插入片段。这些克隆被使用 大学内外的调查人员对结构进行研究 以及导致各种一般疾病的基因的功能。这 克隆库的规模和使用的持续增长威胁着我们 管理图书馆和应对新研究机会的能力。 我们建议开发一个全面的信息管理环境 这将帮助我们充分发挥 YAC 库的潜力。在 构建这个信息管理环境，我们将解决 与其他生物医学直接相关的关键研究问题的数量 面临类似信息管理危机的研究实验室： 1. 我们将制定YAC文库筛选的详细说明 用作综合 YAC 克隆概念模型的实验 代表整个实验室范围的信息系统 活动。 2. 我们将设计一个概念数据模型和一个正式的数据库模式 YAC 图书馆信息系统，以便技术和方法上 YAC 克隆方法的变化可以很容易地纳入其中。这是一个 信息系统开发中的重大研究问题 传统数据模型假设数据库中表示的“世界” 是相对静态的。由于遗传学的快速进步 研究方法，我们的数据模型必须足够灵活 纳入新的发展。 3.我们将实施全面的数据库管理系统，派生 来自正式数据模型，以支持正在进行的 YAC 研究活动。 该系统将通过“序列标记”来识别克隆和引物对 站点”（STS），它将促进合作研究安排 通过跟踪已发送到外部或从外部接收的克隆 研究人员并通过记录这些提供的 YAC 结构信息 调查人员。 4. 我们将开发一个强大的数据采集环境，以便实验室 人员可以将实验结果直接输入信息中 系统。目标是消除容易出错的转录的需要 的实验结果。 由遗传学家和医学计算机科学家组成的研究团队 将通过创建最先进的综合性 YAC 来实现这些目标 信息管理环境是可访问的、有用的、可靠的、 并响应技术变革，这最终可能成为 更全面的“分子遗传学”的核心组成部分 工作站。”