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CHOLESTEROL INTERACTIONS: SERUM AND MEMBRANE PROTEINS

胆固醇相互作用：血清和膜蛋白

基本信息

批准号：
3337438
负责人：
PHILIP L YEAGLE
金额：
$ 6.89万
依托单位：
STATE UNIVERSITY OF NEW YORK AT BUFFALO
依托单位国家：
美国
项目类别：
财政年份：
1979
资助国家：
美国
起止时间：
1979-04-01 至 1986-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3337438
关键词：
atherosclerosis blood proteins cholesterol circular dichroism erythrocyte membrane human tissue low density lipoprotein membrane structure molecular pathology nuclear magnetic resonance spectroscopy phospholipids

项目摘要

This project explores the largely unexplored concept of cholesterol interaction with proteins. Some indirect measures have suggested that such interactions may be important in the structure of human low density lipoprotein (LDL) and band 3 protein (anion transport protein) from the human erythrocyte membrane. Because of the roles of LDL and cholesterol in the pathogenesis of atherosclerosis, it is important to explore the behavior of cholesterol in two of its locations in human blood, low density lipoproteins and the erythrocyte membrane. Discovery of an interaction with protein would significantly alter the common view of cholesterol behavior in membranes and lipoproteins. This proposal probes by direct, non-perturbing methods (for the first time) and new indirect and somewhat perturbing methods the possible interaction of cholesterol with protein in: 1) LDL, and 2) the erythrocyte membrane. Two proteins from the erythrocyte membrane will be examined; MN-glycoprotein (glycophorin) and band 3 protein. 31P NMR, 2D NMR, ESR and CD measurements will be explored. The results will be employed to describe more fully the structure of LDL and the erythrocyte membrane, and lipid-protein interactions in these systems. The information should prove valuable to the understanding of the transport of cholesterol and the development of the placques of atherosclerosis.

这个项目探索了很大程度上未被探索的胆固醇相互作用的概念。用蛋白质。一些间接措施表明，这种相互作用可能在人类低密度脂蛋白和带3的结构中起重要作用来自人红细胞膜的蛋白质(阴离子转运蛋白)。因为关于低密度脂蛋白和胆固醇在动脉粥样硬化发病机制中的作用，它对于探索胆固醇在两个位置的行为很重要人体血液、低密度脂蛋白和红细胞膜。发现与蛋白质的相互作用将极大地改变人们对膜和脂蛋白中的胆固醇行为。本提案采用直接的、非干扰的方法(首次)和新的间接和有些微扰的方法，可能的相互作用胆固醇和蛋白质在：1)低密度脂蛋白，和2)红细胞膜。二红细胞膜的蛋白质将被检测；MN-糖蛋白 (血糖素)和带3蛋白。31P核磁共振、2D核磁共振、ESR和CD测量将被探索。这些结果将被用来更全面地描述低密度脂蛋白的结构和红细胞膜，以及这些系统中的脂-蛋白相互作用。这个信息应该被证明是对理解胆固醇与动脉粥样硬化安慰剂的发展。