Multi-object, high-throughput, spectro-microscopy

多目标、高通量、光谱显微镜

• 批准号: EP/H008632/1
• 负责人: John Girkin
• 金额: $ 57.53万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FH008632%2F1
• 关键词: Multi; object; throughput; spectro; microscopy

Microscopy is a pervasive technology with applications from biology to material and nanoscience. At their heart, microscopes are predominately based on high numerical aperture, short focal length objective lenses producing images that can be seen by eye and/or electronically recorded, typically with 3-colour CCD cameras or a selection of coloured filters, but leaving the full spectral details unknown.We plan to revolutionise the field of spectroscopic microscopy by developing a technology that will provide full colour spectrum, simultaneously from many points within a sample. To record high resolution (spatially and spectral) spectra across an extended field of view with a conventional microscope one currently employs a time-sequential recording technique: either a spectrally dispersed image of a single point or a line is scanned across the scene, or an extended image is recorded through a spectrally scanned filter, both methods being comparatively slow. We propose to develop a novel approach combining all the advantages of the existing methods into a single unit: i.e. a system with both high spatial, spectral, and temporal resolution. Although real-time, high-resolution spectral imaging has been a goal for many years no existing approach comes close to this combination of features. Of those techniques that record spectral and image data simultaneously, the Computed Tomographic Imaging Spectrometer (CTIS) is of most interest. However our technique is superior because it can view the whole field indiscriminately, rather than specific regions, it is has a superior signal to noise ratio, and, most importantly, it has ~1000 spectral channels as opposed to ~50. This project will develop a demonstrator and benchmark it against challenging problems in bio microscopy, spectroscopy and security, including multipoint SERS and Raman microscopy, and spectroscopically contrasted imaging in a range of biological samples.

显微镜是一种普遍的技术，其应用范围从生物学到材料和纳米科学。显微镜的核心是高数值孔径、短焦距的物镜，这些物镜可以产生肉眼可见的图像和/或电子记录的图像，通常使用三色CCD相机或彩色滤光片，但不知道完整的光谱细节。我们计划通过开发一种技术来彻底改变光谱显微镜领域，该技术将提供完整的彩色光谱，同时从样本中的多个点。为了记录高分辨率（空间和光谱）光谱在一个扩展的视野与传统的显微镜，目前采用的时间顺序记录技术：无论是一个光谱分散的图像的一个单一的点或线扫描整个场景，或扩展的图像记录通过光谱扫描过滤器，这两种方法是比较慢。我们建议开发一种新的方法，将现有方法的所有优点结合到一个单元中：即具有高空间，光谱和时间分辨率的系统。虽然实时，高分辨率的光谱成像多年来一直是一个目标，没有现有的方法来接近这种组合的功能。在同时记录光谱和图像数据的那些技术中，计算机断层成像光谱仪（CTIS）是最感兴趣的。然而，我们的技术是上级的，因为它可以不加区别地查看整个领域，而不是特定区域，它具有上级信噪比，最重要的是，它具有~1000个光谱通道，而不是~50个。该项目将开发一个演示器，并将其与生物显微镜，光谱学和安全性方面的挑战性问题进行基准测试，包括多点Sers和拉曼显微镜，以及一系列生物样品的光谱对比成像。

项目成果

Impact of wavefront distortion and scattering on 2-photon microscopy in mammalian brain tissue.

• DOI: 10.1364/oe.19.022755
• 发表时间: 2011-11-07
• 期刊: Optics express
• 影响因子: 3.8
• 作者: Chaigneau E;Wright AJ;Poland SP;Girkin JM;Silver RA
• 通讯作者: Silver RA

Micro-endoscope for in vivo widefield high spatial resolution fluorescent imaging.

• DOI: 10.1364/boe.3.001274
• 发表时间: 2012-06-01
• 期刊: Biomedical optics express
• 影响因子: 3.4
• 作者: Saunter CD;Semprini S;Buckley C;Mullins J;Girkin JM
• 通讯作者: Girkin JM

Multidepth, multiparticle tracking for active microrheology using a smart camera

使用智能相机进行主动微流变学的多深度、多粒子跟踪

• DOI: 10.1063/1.3567801
• 发表时间: 2011
• 期刊: Review of Scientific Instruments
• 影响因子: 1.6
• 作者: Silburn S

From structure to function: mitochondrial morphology, motion and shaping in vascular smooth muscle.

• DOI: 10.1159/000353883
• 发表时间: 2013
• 期刊: Journal of vascular research
• 影响因子: 1.7
• 作者: McCarron JG;Wilson C;Sandison ME;Olson ML;Girkin JM;Saunter C;Chalmers S
• 通讯作者: Chalmers S

Improved method for kinetic studies in microreactors using flow manipulation and noninvasive Raman spectrometry.

• DOI: 10.1021/ja1102234
• 发表时间: 2011-03
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Sergey Mozharov;A. Nordon;D. Littlejohn;C. Wiles;P. Watts;Paul H. Dallin;J. Girkin