TRANSPORT ACROSS ALVEOLAR CAPILLARY MEMBRANE

穿过肺泡毛细血管膜的运输

• 批准号: 3336930
• 负责人: AUBREY E. TAYLOR
• 金额: $ 13.85万
• 依托单位: UNIVERSITY OF SOUTH ALABAMA
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-09-01 至 1987-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3336930
• 关键词: capillary; hydrostatic pressure; lung alveolus; membrane permeability; osmotic pressure; pulmonary edema; vascular endothelium permeability

The major objective of this research proposal is to study the Starling forces i.e., (tissue pressure, capillary pressure, tissue colloid osmotic pressure, capillary osmotic pressure and lymph flow) and the permeability properties of the pulmonary exchange vessels during the formation of pulmonary edema induced by increased hydrostatis pressure. An in situ dog lung can be continuously weighed and arterial and venous pressures can be controlled at any desired level. A small lymphatic vessel will also be cannulated to measure flow and lymph protein as capillary pressure is elevated. Capillary pressure will be elevated in steps of 5 mm Hg and the capillary filtration coefficient, lymphatic protein flux (lymph flow times lymph protein concentration), tissue fluid pressure (as measured by implanted capsules and small fluid filled airways), capillary pressure and lymph and plasma colloid osmotic pressure (measured using a membrane osmometer) will be evaluated at each new steady state obtained by the lung. The permeability characteristics of the pulmonary exchange vessels can be assessed at each pressure level by estimating the reflection coefficient and permeability surface area products of 7 different protein fractions in lymph. In addition, the osmotic transient approach will be used as an additional estimate of capillary permeability. By combining an analysis of Starling forces changes with capillary permeability measurements, we will be able to build a more comprehensive model of the development of pulmonary edema caused by other agents such as histamine, serotonin, etc.

这项研究计划的主要目标是研究Starling部队 也就是说，（组织压力，毛细血管压力，组织胶体渗透压， 毛细血管渗透压和淋巴流）和渗透性特性 肺水肿形成过程中的肺血管交换 通过增加流体静力学压力。 原位狗肺可以连续地 并且动脉和静脉压力可以被控制在任何期望的 水平 小淋巴管也将被插管以测量流量， 淋巴蛋白作为毛细血管压力升高。 毛细管压力将是 升高5 mm Hg的步骤和毛细血管过滤系数，淋巴 蛋白通量（淋巴液流量乘以淋巴蛋白浓度），组织液 压力（通过植入的胶囊和充满液体的小气道测量）， 毛细血管压和淋巴液及血浆胶体渗透压（使用 膜渗透压计）将在通过 肺。 肺交换血管的渗透性特征可以是 通过估计反射系数在每个压力水平下进行评估， 淋巴液中7种不同蛋白质组分的渗透性表面积乘积。 此外，渗透瞬变方法将被用作额外的 毛细血管渗透性的估计。 结合Starling力变化与毛管渗透率的分析， 测量，我们将能够建立一个更全面的模型， 发展由其他药剂如组胺引起的肺水肿， 血清素等。