CNS SEROTONERGIC NEURONS AND BREATHING

我们对控制呼吸的中枢神经通路的了解是 不完整 此外，关于哪些神经递质是 几乎不存在。 在雇用新的 一种结合免疫组织化学和荧光染料示踪的方法 我们发现了中缝和 膈运动核 我们还发现了血清素TRH 以及膈神经核的P物质 电刺激 中缝或1. v.完全停止给予5-羟色胺激动剂 正常呼吸和消除自发膈神经活动。 我们 建议将细胞内和细胞外电生理联合收割机 免疫组织化学和染料示踪技术来证实这一点 中缝-膈核通路 具体来说，我们将确定 这条通路在正常呼吸中的重要性以及它是如何通过 其他输入到呼吸中心。 此外，我们将雇用 药理学试剂，以确定血清素，TRH和 P物质在这条通路中。 最后，我们将确定是否有 这些神经元中的递质共存。 中央的知识 膈神经核的输入对于理解正常的 呼吸的过程，但在理解这种疾病的关键 系统 呼吸系统的中枢缺陷导致肌肉 疲劳可能与慢性阻塞性呼吸道疾病有关， 中枢性睡眠呼吸暂停和婴儿猝死综合症。 确定 神经投射和神经递质参与控制 呼吸应该提供新的信息， 了解可能导致这些疾病的神经缺陷 并可能为开发新的治疗方法提供基础， 这些呼吸系统疾病。

Our knowldege of the central neuropathways controlling respiration is incomplete. In addition, information regarding which neurotransmitters are involved in these pathways is virtually non-existent. In employing a new approach which combines immunohistochemistry with fluorescent dye tracing techniques we found evidence for a neural connection between the raphe and phrenic motor nucleus. We also localized the presence of serotonin, TRH and Substance P at the phrenic nucleus. Electrical stimulation of the raphe or 1. v. administration of a serotonin agonist completely stopped normal respiration and abolished spontaneous phrenic nerve activity. We propose to combine intracellular and extracellular electrophysiological techniques with immunohistochemistry and dye tracing to confirm this raphe-phrenic nucleus pathway. Specifically, we will determine the importance of this pathway in normal breathing and how it is modulated by other inputs into the respiratory centers. Also, we will employ pharmacological agents to determine the role of serotonin, TRH and Substance P in this pathway. Finally, we will determine if there is co-existence of transmitters in these neurons. Knowledge of the central inputs into the phrenic nucleus is important in understanding the normal process of breathing, but is critical in understanding disorders of this system. Central defects of the respiratory system resulting in muscle fatigue may be associated with chronic obstructive respiratory diseases as well as Central Sleep Apnea and Sudden Infant Death Syndrome. Determining the neural projections and neurotransmitters involved in the control of respiration should provide new information that will increase our understanding of the neural defects that can contribute to these disease states, and may offer a basis for developing new therapies for correcting these respiratory disorders.