NMR STUDIES IN NORMAL AND DISEASES HUMAN TISSUES

31 P NMR 提供了一种独特的非侵入性肌肉生物化学方法 正常和患病患者。 10 1/4" 通孔核磁共振波谱仪 提供了评估四肢肌肉生物化学的机会 正常人和患病的人。 肌肉生物能学的主要变化 在方便的运动范围内，磷酸肌酸 (PCr) 会降低 从大约 ATP 水平的 6 倍到 APT 水平的一倍 无机磷酸盐的相应上升实际上保持不变。 PCr/P i （或 PCr/(PCr P i )）评估 休息和锻炼的人类前臂和小腿肌肉，并评估 向组织输送氧气的能力和线粒体的能力 动员底物并利用氧气维持高 PCr 水平 且 P i 低。 31 P NMR 研究中误差性质的研究 此处的人体组织应用于休息时的人体腿部， 正常对照组和单侧患者的运动条件 周围血管疾病。 数据的重复性和可靠性 在这些条件下的这些群体将为使用提供基线 核磁共振作为临床分析生化工具；腿是器官 最适用于各种疾病状态，并且这些数据将提供 未来应用于其他身体器官的基线。 因此协议 涉及适当的休息状态，最大限度地锻炼肢体 特定受试者，正常或患病，然后插入肢体 定量练习后立即进入核磁共振波谱仪 养生法。 建立了该程序的恢复时间类别 并确定了它们的可靠性。 在此数据库上，NMR 可靠性将 通过适当的统计方法建立，并与 从其他方法获得的疾病类别。 课程预测 周围血管疾病的发生及其治疗效果 核磁共振技术的程序最终可能是可能的。

31P NMR presents a unique approach to non-invasive muscle biochemistry in normal and diseased patients. A 10 1/4" clear bore NMR spectrometer affords an opportunity to evaluate muscle biochemistry in the limbs of normal and diseased humans. The principal changes of muscle bioenergetics over the convenient range of exercise are phosphocreatine (PCr) decrease from approximately 6 times the ATP level to one times the APT level with corresponding rises of inorganic phosphate remaining practically constant. PCr/Pi (or PCr/(PCr + Pi)) assesses the biochemical function of the resting and exercised human forearm and calf muscle and evaluates the competency of oxygen delivery to tissue and the capability of mitochondria to mobilize substrates and utilize oxygen for maintaining PCr levels high and the Pi low. A study of the nature of errors in the 31P NMR study of human tissues is here applied to the human leg under resting and exercise conditions in normal controls and in patients having unilateral peripheral vascular disease. The repeatability and reliability of the data from these groups under these conditions will afford a baseline for the use of NMR as a clinical analytical biochemical tool; the leg being the organ most available in a variety of diseased states, and the data will afford a baseline for future applications to other body organs. Thus the protocols involve appropriate resting states, maximum limb exercise feasible for the particular subject, normal or diseased, followed by insertion of the limb into the NMR spectrometer immediately following a quantitative exercise regimen. Categories of recovery times from this procedure are established and their reliability determined. On this data base, NMR reliability will be established by appropriate statistical methods, with correlations with disease categories obtained from other methods. Prediction of the course of peripheral vascular disease and the effectiveness of therapeutic procedures by the NMR technique may ultimately be possible.