CATECHOLAMINES AND SEROTONIN IN BLOOD PRESSURE CONTROL

已知5-羟色胺和儿茶酚胺在 调节血压。5-羟色胺激动剂，α-2受体 激动剂和多巴胺激动剂都被证明能降低血液 在高血压动物身上。患有高血压的人通常 用可乐定和α-甲基多巴治疗，这两种药物 中枢刺激α-2降低血压 肾上腺素受体。尽管这些药物在治疗上取得了成功， 还没有关于单个脑干核团如何 使用去甲肾上腺素、肾上腺素、多巴胺和5-羟色胺 血压的变化。随着体内新技术的出现 电化学，我们已经能够跟踪时间进程 用药期间儿茶酚和5-HIAA的持续浓度 诱发性高血压和低血压。在第一次获奖期间 在这笔赠款期间，我们发现5-羟色胺代谢物5- 当血压升高时，HIAA在大多数核团中都会增加。 增加了。儿茶酚的峰值一般在 高血压。我们发现，只有在c1核中才有 儿茶酚和吲哚的电化学峰互换 与血压有关的高血压和低血压。 在初步实验中，我们还发现邻苯二酚 下丘脑外侧峰与下丘脑外侧峰呈负相关和线性相关 与血清渗透压有关。在这笔补助金的续期中，我们将研究 孤束核的儿茶酚和吲哚代谢 蓝斑，C_1和A_1区，疑核， 室旁核、视前区和外侧核 下丘脑在血清渗透压和循环变化中的作用 音量。除了体内的电化学，我们还将在 活体透析研究去甲肾上腺素和肾上腺素的释放 核内药物引起的血压变化。的效果 将比较Spraogue Dawley和SpragueDawley的渗透变化 加压素缺乏Brattleboro大鼠。加压素的能力 在NTS中重置压力感受器增益和降低儿茶酚将是 比较血管加压素输注对儿茶酚和苯丙氨酸的影响 在其他脑干核团中释放吲哚。这些实验 应该描述单胺类物质在个体中的作用 脑干核团在维持血压、血清渗透压、 和循环量。这些研究的结果已经表明 治疗高血压的新疗法。5-羟色胺激动剂和α-2 激动剂可能是一种有效的抗高血压药物。

Serotonin and the catecholamines are known to play a role in the regulation of blood pressure. Serotonin agonists, alpha-2 receptor agonists and dopamine agonists have all been shown to lower blood in hypertensive animals. Humans with hypertension are routinely treated with clonidine and alpha methyldopa, two drugs which lower blood pressure by central stimulation of alpha-2 adrenoreceptors. Despite the therapeutic success of these drugs, there is no detailed knowledge of how individual brainstem nuclei use norepinephrine, epinephrine, dopamine and serotonin during changes in blood pressure. With the new technique of in vivo electrochemistry, we have been able to follow the time course of catechol and 5-HIAA concentrations continuously during drug- induced hypertension and hypotension. During the first award period of this grant, we found that the serotonin metabolite 5- HIAA was increased in most nuclei whenever blood pressure was increased. The catechol peak was generally reduced during hypertension. We found that only in the C1 nucleus were the electrochemical peaks for catechols and indoles reciprocally related to blood pressure for both hypertension and hypotension. In preliminary experiments, we have also found that the catechol peak in the lateral hypothalamus is inversely and linearly related to serum osmolarity. In the renewal of this grant we will study catechol and indole metabolism in nucleus tractus solitarius, locus coeruleus, the C1 and A1 areas, the nucleus ambiguous, the paraventricular nucleus, the preoptic area and lateral hypothalamus during changes in serum osmolarity and circulating volume. In addition to in vivo electrochemistry, we will use in vivo dialysis to study norepinephrine and epinephrine release in C1 nucleus during drug induced changes in blood pressure. Effects of osmotic changes will be compared in Sprague Dawley and vasopressin deficient Brattleboro rats. The ability of vasopressin to reset baroreceptor gain and lower catechols in NTS will be compared with the effect of vasopressin infusions on catechol and indole release in other brainstem nuclei. These experiments should characterize the role of monoamines in individual brainstem nuclei in maintaining blood pressure, serum osmolarity, and circulating volume. Results of these studies already suggest a new therapy for hypertension. Serotonin agonists and alpha-2 agonists may make a potent antihypertensive combination.