PROBING THE MECHANICAL CONTROL OF STEM CELL FATE THROUGH THE DEVELOPMENT OF NOVEL, NON-INVASIVE IMAGING TECHNOLOGIES

通过开发新型非侵入性成像技术探索干细胞命运的机械控制

• 批准号: EP/H045848/1
• 负责人: Phil Stephens
• 金额: $ 196.9万
• 依托单位: CARDIFF UNIVERSITY
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2010
• 资助国家: 英国
• 起止时间: 2010 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FH045848%2F1
• 关键词: PROBING; MECHANICAL; CONTROL; STEM; CELL

Whilst in our increasing ageing population stem cell science and technology holds a great deal of promise within the context of tissue repair and regeneration, moving this technology to the clinics has been relatively slow due to a number of distinct 'barriers'. For example, whilst we know a lot about the function and response of stem cells in the laboratory, we know very little about their behaviour in tissues within individuals. A further major barrier has been the inability to accurately track cell lineages and to distinguish them from other cell types within the tissue (i.e. when one cell divides to become two cells are they the same or different? Is this effect the same or different each time the cell divides? Where do they go?). This application will address these issues by bringing together researchers across different scientific disciplines in the physical and life sciences to develop novel technologies for stem cell science. Specifically, we will develop new ways of non-destructively labelling stem cells by manipulating molecules within the cells so we can follow both their position and their eventual fate (i.e. what do these stem cells turn into?). In order to image the cells we will develop new microscopic techniques that allow us to view these cells in a non-invasive, non-harmful way (unlike current approaches) and we will utilise technologies that will eventually enable us to image these cells deep within patient tissues. Being able to follow these stem cells will allow us to examine the mechanical influence of their surrounding tissue environments. Armed with such knowledge we will mechanically manipulate the surrounding environment to direct stem cells into our tissue of choice in order to deliver custom designed tissues on demand (either within the laboratory or eventually within a patient). Overall, our ultimate aim is to develop new tools to allow us to investigate and control stem cell biology in order to realise the true clinical potential of these cells.

虽然在我们日益老龄化的人口中，干细胞科学技术在组织修复和再生方面具有很大的前景，但由于一些明显的“障碍”，将这项技术转移到诊所的速度相对较慢。例如，虽然我们在实验室中对干细胞的功能和反应了解很多，但我们对它们在个体组织中的行为知之甚少。另一个主要障碍是无法准确地追踪细胞系并将它们与组织内的其他细胞类型区分开来（即当一个细胞分裂成两个细胞时，它们是相同的还是不同的？）每次细胞分裂时，这种效应是相同的还是不同的？它们去了哪里？)。该应用程序将通过汇集物理和生命科学领域不同学科的研究人员来开发干细胞科学的新技术来解决这些问题。具体来说，我们将通过操纵细胞内的分子来开发非破坏性标记干细胞的新方法，这样我们就可以跟踪它们的位置和最终命运（即，这些干细胞变成了什么？）。为了给细胞成像，我们将开发新的显微技术，使我们能够以一种非侵入性、无害的方式观察这些细胞（与目前的方法不同），我们将利用最终使我们能够在患者组织深处对这些细胞成像的技术。能够跟踪这些干细胞将使我们能够检查它们周围组织环境的机械影响。有了这些知识，我们将机械地操纵周围环境，将干细胞引导到我们选择的组织中，以便根据需要提供定制设计的组织（无论是在实验室还是最终在患者体内）。总的来说，我们的最终目标是开发新的工具，使我们能够研究和控制干细胞生物学，以实现这些细胞的真正临床潜力。

项目成果

Integration-Free Reprogramming of Lamina Propria Progenitor Cells.

固有层祖细胞的无整合重编程。

• DOI: 10.1177/0022034516637579
• 发表时间: 2016
• 期刊: Journal of dental research
• 影响因子: 7.6
• 作者: Howard-Jones RA

CARS drives new microscopy technique

CARS 推动新的显微镜技术

• 发表时间: 2014
• 作者: Langbein, W

Assessment of the stress relaxation characteristics of critical gels formed under unidirectional shear flow by controlled stress parallel superposition rheometry

通过受控应力平行叠加流变仪评估单向剪切流下形成的临界凝胶的应力松弛特性

• DOI: 10.1016/j.jnnfm.2014.12.004
• 发表时间: 2015
• 期刊: Journal of Non-Newtonian Fluid Mechanics
• 影响因子: 3.1
• 作者: Curtis D

Hyperspectral volumetric coherent anti-Stokes Raman scattering microscopy: quantitative volume determination and NaCl as non-resonant standard.

高光谱体积相干反stokes拉曼散射显微镜：定量体积测定和NaCl作为非谐波标准。

• DOI: 10.1002/jrs.4876
• 发表时间: 2016-09
• 期刊: JOURNAL OF RAMAN SPECTROSCOPY
• 影响因子: 2.5
• 作者: Karuna, Arnica;Masia, Francesco;Borri, Paola;Langbein, Wolfgang
• 通讯作者: Langbein, Wolfgang

A 3D ex vivo mandible slice system for longitudinal culturing of transplanted dental pulp progenitor cells.

• DOI: 10.1002/cyto.a.22680
• 发表时间: 2015-10
• 期刊: Cytometry. Part A : the journal of the International Society for Analytical Cytology
• 作者: Colombo JS;Howard-Jones RA;Young FI;Waddington RJ;Errington RJ;Sloan AJ
• 通讯作者: Sloan AJ