BLOOD VESSEL: FUNCTION TRANSMITTER LOCALIZATION

一直在努力验证脑血管疾病的性质， 神经支配和这些神经产生的影响。 四个假定 发射机已被明确证明是存在于墙上的 脑血管：去甲肾上腺素、乙酰胆碱、P物质和 血管活性肠肽 我们建议继续扩大我们的 调查这些发射机的作用，使用各种 组织化学、体外和生物化学技术。 的具体目标 这些建议是：1. 定位每个发射机， 免疫组化在光镜和电镜水平。 2. 调查神经源和共传播的可能性， 选择性破坏特定类型的神经， 化学上 3. 验证神经活动并测试可能性 通过测量体外释放的递质。 4. 表征和定位这些递质的受体， 放射性配体结合技术和光学显微镜放射自显影。 5. 通过测量来研究这些变送器的功能作用 收缩反应和氨基酸转运在体外前后 选择性神经元破坏 这种多方面的方法使我们能够 首先验证手术或化学治疗的有效性和特异性， 神经破坏 随后将进行血管检查 功能，包括平滑肌收缩和氨基酸摄取。 建立可靠的神经破坏方法也将使 有可能进一步研究这些神经的作用， 方法. 增加感觉和运动神经支配的知识， 探索不同的功能之间可能的相互关系， 神经的种类可以提供对发病机制的理解 脑血管疾病，如中风，头痛和后遗症 脑外伤，以及提出新的治疗方法。

There has been an ongoing effort to verify the nature of cerebrovascular innervation and the effects these nerves produce. Four putative transmitters have been definitely shown to be present in the wall of cerebral blood vessels: norepinephrine, acetylcholine, substance P and vasoactive intestinal peptide. We propose to continue to expand our inquiry into the role of these transmitters using a variety of histochemical, in vitro and biochemical techniques. The specific aims of this proposal are: 1. Localize each transmitter using immunohistochemistry at the light and electron microscopic levels. 2. Investigate the source of nerves and the possibility of cotransmission by selective destruction of specific types of nerves either surgically or chemically. 3. Verify the activity of nerves and test for the possibility of corelease of transmitters by measuring transmitter release in vitro. 4. Characterize and localize receptors for these transmitters using radioligand binding techniques and light microscopic autoradiography. 5. Investigate the functional role of these transmitters by measuring contractile response and amino acid transport in vitro before and after selective neuronal destruction. This multifaceted approach allows us to first verify the effectiveness and specificity of surgical or chemical nerve destruction. This will be followed by tests of blood vessel function, both smooth muscle contraction and amino acid uptake. Establishment of reliable methods for destruction of nerves will also make it possible to further investigate the role of these nerves using in vivo methods. Increased knowledge of both sensory and motor innervation and exploration of possible functional interrelationships between different kinds of nerves may provide understanding of the pathogenesis of cerebrovascular disorders, such as stroke, headache and the sequelae of cerebral trauma, as well as suggest new approaches for therapy.