NEURAL CORRELATES OF VOCAL LEARNING

使用~3H-胸腺嘧啶核苷的放射自显影研究表明，新的神经元是 加入成年金丝雀腹侧纹状体尾部(HVC) 大脑。HVC位于前脑，是歌曲控制系统的一部分 在歌曲学习方面。新的神经元是由脑室分裂而形成的。 将细胞分区，然后迁移、分化并连接到现有的 电路。计划在未来5年进行的研究将描述 在这个系统中神经元募集的动力学，控制因素 它，以及新神经元取代旧神经元的可能性。 这项工作将继续使用~3H-胸腺嘧啶核苷和放射自显影，因为 以及硅胶植入的类固醇激素，通过移除两者而震耳欲聋 耳蜗骨和红藻氨酸损伤。神经发生和发育的意义 成人HVC的神经元替代将通过搜索颞叶进行研究 这些现象与成人一年中的不同时期之间的相关性 金丝雀特别容易学习新的歌曲音节，忘记旧的 一个。这里检验的假设是，在 歌曲学习、遗忘和替换的时间发生 下丘脑的神经元。行为研究将涉及歌曲录制和 声谱分析。在这种情况下，我们的具体目标将 BE：1.测量歌曲知觉记忆和运动记忆的半衰期 成年雄性金丝雀，以及它们对激素的依赖程度。 2.确定神经发生和神经元发生的季节性 成年金丝雀的更替及其与时间的关系 歌声不稳，忘却与学习。3.确定在多大程度上， 如果有的话，激素和经验会影响神经发生和神经元 在HVC中更换。4.确定新HVC的生存曲线 神经元，以及它们在不同神经元之间的差异程度 上课。5.确定新的HVC神经元在迁移和迁移过程中的定向 找个地方工作。6.确定神经元招募的程度 发生在从孵化到性成熟的时期，所以图案 招聘(和替补？)当时发生的事情可以比较 与那些发生在成年期的。7.干扰神经元 成人HVC的招募和神经元替代研究对其的影响 新歌的记忆保持和学习。 对神经发生和再生障碍性疾病发生及意义的认识 成人大脑中的神经元替代可能对 神经学练习。

Autoradiographic studies using 3H-thymidine indicate that new neurons are added to hyperstriatum ventralis, pars caudalis (HVc) of the adult canary brain. HVc is in the forebrain and is part of the song control system used in song learning. The new neurons are formed by division of ventricular zone cells, then migrate, differentiate and become connected to existing circuitry. Research planned for the next 5 years will describe the dynamics of neuronal recruitment in this system, the factors that control it, and the possibility that the new neurons replace older neurons. 3H-thymidine and autoradiography will continue to be used for this work, as well as silastic implants of steroid hormones, deafening by removal of both cochleas, and kainic acid lesions. The significance of neurogenesis and neuronal replacement in adult HVc will be studied by searching for temporal correlations between these phenomena and times of year during which adult canaries are particularly prone to learn new song syllables and forget old ones. The hypothesis tested here is that there is a correlation between the temporal occurrence of song learning, song forgetting and replacement of HVc neurons. The behavioral studies will involve song recording and sound-spectrographic analysis. Within this scenario our specific aims will be: 1. To measure the half-life of song perceptual and motor memories in adult male canaries, and the extent to which they are hormone dependent. 2. To determine the seasonal occurrence of neurogenesis and neuronal replacement in adult canaries, and their temporal relation to periods of song instability, forgetting and learning. 3. To determine to what extent, if any, hormones and experience influence neurogenesis and neuronal replacement in HVc. 4. To determine the survival curves of new HVc neurons, and the extent to which they may vary between different neuronal classes. 5. To determine how new HVc neurons orient during migration and find a place to work. 6. To determine to what extent neuronal recruitment occurs during the period from hatching to sexual maturity, so that patterns of recruitment (and replacement?) occurring at that time can be compared with those occurring in adulthood. 7. To interfere with neuronal recruitment and neuronal replacement in adult HVc to see how this affects memory retention and learning of new songs. An appreciaton of the occurrence and significance of neurogenesis and neuronal replacement in adult brain could have profound effects on neurological practice.