TRIGEMINAL MODULATION OF AUTONOMIC BEHAVIOR

自主行为的三叉神经调节

• 批准号: 3354774
• 负责人: W MICHAEL PANNETON
• 金额: $ 8.15万
• 依托单位: SAINT LOUIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3354774
• 关键词: autonomic reflex; brain stem; cardiovascular function; electrostimulus; gamma aminobutyrate; laboratory rat; lidocaine; microelectrodes; nasopharynx; neuroanatomy; neuropharmacology; neurotransmitters; respiratory function; sensorimotor system; sudden infant death syndrome; trigeminal nerve

Stimulating the nasal cavity exerts a powerful and abrupt alteration of respiratory and cardiovascular rhythms. This remarkable behavior is present in all vertebrates, including man, and commonly is called the diving reflex. The control is mediated through the central nervous system, which possesses the neuronal circuits directing cardiorespiratory function. However, no information is available regarding the specific central neural circuits driving this behavior. My long-term objective is to establish the brainstem reflex pathways utilized in the diving reflex in mammals. The general aim of this proposal is to establish the trigeminal component of these circuits; it specifically aims to 1) determine the locus within the trigeminal sensory complex which receives sensory fibers from the nose and upper respiratory passages using transganglionic transport methods; 2) validate the importance of this area as the trigeminal mediator of cardiorespiratory function using both reversible blocking agents (lidocaine and muscimol) and electrical and chemical (glutamate) stimulation; 3) determine the projections of neurons in this area to brainstem autonomic nuclei using conventional neuroanatomical techniques (HRP, PHA-L, tritiated amino acids); 4) specifically determine the role of the trigeminoparabrachial pathway in the response using blocking agents and if this pathway uses opiates as a chemical messenger (double labeling techniques and naloxone injections). The powerful inhibition of respiration and heart resulting from upper respiratory stimulation may provide a neurogenic basis for the tragedy of the Sudden Infant Death Syndrome (SIDS) and the cardiorespiratory alterations seen in simple intubation procedures. Understanding the brainstem circuitry of the diving response could aid in the diagnosis and remedy of these problems. Knowledge of these same brainstem circuits will serve as a substrate for future investigations of how a simple somatic stimulus could be designed to modulate autonomic behavior and how higher levels of the brain (i.e. limbic) influence cardiorespiratory activity usually controlled at lower levels.

刺激鼻腔会发挥强大而突然的 呼吸道和心血管节奏的改变。 这 包括人在内的所有脊椎动物中都存在着出色的行为 通常称为潜水反射。 控制是介导的 通过具有神经元的中枢神经系统 指挥心肺功能的电路。 但是，不 有关特定中央神经的信息 电路推动这种行为。 我的长期目标是 建立潜水中使用的脑干反射途径 哺乳动物的反射。 该提议的一般目的是 建立这些电路的三叉成分；它 特别是针对1）确定三叉神经中的基因座 感官复合物从鼻子接收感官纤维， 上呼吸道通道使用跨气离子运输 方法; 2）验证该区域的重要性作为三叉神经 使用两种可逆的心肺功能的介体 阻塞剂（利多卡因和麝香酚）和电气和电气 化学（谷氨酸）刺激； 3）确定预测 使用该区域的神经元使用脑干自主神经核使用 常规的神经解剖技术（HRP，PHA-L，Tritived 氨基酸）; 4）明确确定 响应中使用阻止 代理商，如果此途径使用鸦片作为化学信使 （双重标记技术和纳洛酮注射）。 呼吸和心脏的强大抑制 上呼吸道刺激可能为神经源提供 突然的婴儿死亡综合征（SIDS）和 在简单的插管程序中看到的心肺改变。 了解潜水反应的脑干电路 可以帮助诊断和解决这些问题。 了解这些相同的脑干电路将成为 底物以未来对简单的体细胞的调查 可以设计刺激以调节自主行为和 大脑的水平较高（即边缘）如何影响 心肺活性通常以较低的水平控制。