THE CPA: SOMATIC AND AUTONOMIC SYSTEMS

CPA：躯体和自主系统

• 批准号: 6490745
• 负责人: W MICHAEL PANNETON
• 金额: $ 25.9万
• 依托单位: SAINT LOUIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6490745
• 关键词: afferent nerve; autonomic nervous system; blood pressure; brain mapping; brain stem; cardiovascular function; diving /swimming; electrophysiology; immunocytochemistry; laboratory rat; medulla oblongata; neuroanatomy; neurogenic hypertension; neurons; neurophysiology; neuroregulation; reflex; respiration regulatory center; somatic reflex; stimulus /response; striated muscles; sympathetic nervous system; trigeminal nerve

DESCRIPTION (Verbatim from the application): The long-term goal of my research is to establish the brainstem circuits through which trigeminal neurons modulate cardiovascular and respiratory activity in mammals. The cardiorespiratory depression (the diving response) seen after stimulation of he upper respiratory tract (URT) has served as my model. The aims for this proposal are directed towards elucidating the control of blood pressure, specifically in regard to both nasal and muscle stimulation. We shall focus our attention on the interplay between the caudal pressor area (CPA) and its interaction with the premotor sympathetic bulbospinal neurons of the rostroventrolateral medulla RVLM). Since the CPA is basically undefined in the rat, two of our aims are directed to locating it and demonstrating its connections, especially with the RVLM. Two other aims will define its role in blood pressure regulation in general as well as to nasal nd muscle stimulation. A fifth aim will define the primary afferent termination of sensory fibers innervating the triceps sure muscle in the rat. We propose specifically to 1) localize the CPA in the caudal medulla; 2) define the input/output of the CPA; 3) define the central termination of sensory fibers to the triceps sure muscle; 4) determine the role of the CPA during the diving esponse and muscle stimulation; and, 5) characterize the responses of individual neurons in the CPA to hypertensive, hypotensive, oxious, and nasal and muscle stimuli. The CPA has been defined in variable positions within the caudal medulla depending on the species in question. Aim I specifically will identify its location in the rat. Blood pressure will be recorded after stimulating the caudal medulla; such a procedure should localize the pressor areas there. Two complimentary neuroanatomical methodologies will determine the output/input of the PA, especially to the RVLM, as outlined in Aim II The orthogradely transported tracer biotinylated dextrin amine and the retrogradely transported tracer fluorogold will elucidate projections from/to the CPA. There is some controversy regarding the input o the dorsal horn of primary afferent fibers from muscle. Aim III will use transganglionic transport of HRP to investigate this roblem and correlate this data to that seen in Aim II. In Aim IV, the CPA will be tested to determine its role in controlling blood pressure to both nasal and muscle stimulation. Chemical lesions using ibotenic acid will be made in the CPA and changes in cardiovascular behavior to such stimulation determined. Finally, neurons in the CPA will be monitored electrophysiologically in Aim V and their responses recorded to hypertensive, hypotensive, noxious, and nasal and muscle stimuli. This study will provide the most complete study of the CPA, about which little is known. Stimulation of the URT or large muscles in man causes an increase in sympathetic discharge and hypertension. Understanding the brainstem mechanisms governing he vascular constriction after either of these somatic stimuli may provide a neurological explanation for this. Such knowledge may lead to an understanding of essential hypertension.

描述（逐字化的应用程序）：我的研究的长期目标 是建立三叉神经元的脑干电路 调节哺乳动物的心血管和呼吸活性。这 刺激后发现心肺抑郁症（潜水反应） 上呼吸道（URT）已成为我的模型。目的 提案针对阐明血压的控制， 特别是鼻和肌肉刺激。我们将集中精力 注意尾端压力区域（CPA）之间的相互作用及其之间的相互作用 与运动前交感神经脊髓神经元的相互作用 Rostroventrololal外侧髓质RVLM）。由于CPA基本上是未定义的 老鼠，我们的两个目标被指示定位并证明它 连接，尤其是与RVLM。其他两个目标将定义其在 总体上调节血压以及鼻腔刺激。 第五个目标将定义感官纤维的主要传入终止 将三头肌支撑大鼠的肌肉支配。我们专门针对1） 将CPA定位在尾骨中； 2）定义CPA的输入/输出； 3）定义对三头肌的感觉纤维的中心终止； 4）确定CPA在潜水源和肌肉中的作用 刺激； 5）表征单个神经元中单个神经元的反应 CPA至高血压，降压，氧气，鼻和肌肉刺激。 CPA已被定义为尾骨内的可变位置 取决于所讨论的物种。目的我专门将确定它的 位置在大鼠中。刺激血压将记录 尾髓；这样的程序应将其定位在那里的台阶区域。二 免费神经解剖学方法将确定的输出/输入 PA，尤其是对RVLM的PA，如AIM II中概述的 运输的示踪剂生物素化葡萄蛋白胺和逆转的运输 示踪剂Fluorogold将阐明从/到CPA的投影。有一些 关于输入的争议o主要传入纤维的背角 来自肌肉。 AIM III将使用HRP的透射运输进行调查 这种棘手的数据将这些数据与AIM II中看到的数据相关联。在AIM IV中，CPA 将进行测试以确定其在控制血压中的作用 鼻和肌肉刺激。将使用依靠苯甲酸的化学病变 在CPA中，心血管行为的变化以这种刺激 决定。最后，将监测CPA中的神经元 在AIM V中的电生理学及其反应记录在高血压， 低血压，有害，鼻和肌肉刺激。 这项研究将为CPA提供最完整的研究，这很少 已知。刺激人类中的urt或大肌肉会增加 交感神经和高血压。了解脑干机制 这些躯体刺激中的任何一个都可以管理他的血管收缩 为此提供神经系统解释。这样的知识可能会导致 理解基本高血压。