BIOCHEMISTRY OF PROMINENT AUDITORY STRUCTURES

耳蜗和耳蜗外膜显微标本的定量组织化学研究 将完成耳蜗核。 微量化学荧光法，低 表面荧光温度扫描、辐射测量和GLC质谱 将用于 能量代谢的特点，环核苷酸， 前列腺素代谢的研究将通过全面的 通过直接生化分析表征1. 酶的模式， 2. 中间水平，和3。 产物形成的速率 主要途径。 酶活性、代谢物水平和终产物速率 形成的主要途径可作为生化指标 机制等 简单的实验条件将被用来减少和 增加通过主要途径的流量率， 病理生理学（如缺血、低血糖）和药理学措施 (e.g.碘乙酸盐、水杨酸盐、哇巴因），并研究这些药物的作用。 三个参数的变化。 根据积累的知识 在前几个阶段，将设计实验来评估 传入神经阻滞、生理性音调刺激、噪声创伤的影响 以及耳毒性药物对这些定义参数的影响。 研究将启动， 听觉结构发育的生物化学， 对噪声引发的敏感性。

Quantitative histochemical studies of microscopic samples of the cochlea and the cochlear nucleus will be done. Microchemical fluorimetric procedures, low temperature scanning of surface fluorescence, radiometry and GLC-masspectrometry will be used. The peculiarities of energy metabolism, of cyclic nucleotide, and of prostaglandin metabolism will be investigated by comprehensive characterization through direct biochemical analysis of 1. the enzyme patterns, 2. the intermediate levels, and of 3. the rate of product formation of the major pathways. Enzyme activities, metabolite levels and rates of end product formation of the major pathways can be used as indicators of biochemical mechanisms. Simple experimental conditions will be used to decrease and increase the rate of flux through the major pathways by using established pathophysiological (e.g. ischemia, hypoglycemia) and pharmacological measures (e.g. iodoacetate, salicylate, ouabain) and to study the effect of such alterations on the three defined parameters. Based on the knowledge accumulated in the previous stages, experiments will be designed which will assess the influence of deafferentation, of physiological tone stimulation, of noise trauma and of ototoxic drugs on these defined parameters. Studies will be initiated of the biochemistry of development of auditory structures with special reference to susceptibility to priming by noise.