ISLET CELL BIOLOGY CORE

胰岛细胞生物学核心

• 批准号: 7283872
• 负责人: FRANZ M MATSCHINSKY
• 金额: $ 16.82万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7283872
• 关键词: Cell Nucleus; Cells; Cellular biology; Consultations; Coupling; Depth; Development; Diabetes Mellitus; Diabetic mouse; Disease; Eragrostis; Evolution; Faculty; Fostering; Genes; Glucokinase; Goals; Grant; Health; Hereditary Disease; Hormones; Individual; Industry; Institution; Islet Cell; Islets of Langerhans; Laboratories; Laboratory Research; Leadership; Life; Measurement; Mus; National Institute of Diabetes and Digestive and Kidney Diseases; Numbers; Operative Surgical Procedures; Pancreas; Pathology; Pennsylvania; Phenotype; Play; Positioning Attribute; Procedures; Purpose; Rattus; Research; Research Personnel; Research Support; Resources; Role; Scientist; Sepharose; Services; Signal Pathway; Staging; Stem Cell Research; Stimulus; Subgroup; Talents; Technology; Tissues; Training; Transplantation; Universities; Wolves; Work; base; career; cell growth; concept; cost; design; diabetes mellitus therapy; glucagon-like peptide 1; hormone metabolism; inhibitor/antagonist; interest; islet; islet stem cells; member; metabolic abnormality assessment; metabolomics; programs

ISLET CELL BIOLOGY CORE: Director - F. Matschinsky It is the purpose of this core to assist investigators who currently study or have plans to study independently or collaboratively various aspects of pancreatic islet cell biology. In order to accomplish this goal the Core provides four services. First, it will perform extra corporal phenotyping of the endocrine pancreas of mouse and rat using the intact isolated perfused or minced perifused pancreas. Second, it will isolate, culture, and functionally assess pancreatic islets of rat and mouse including batch incubations, perifusions, respirometry, measurements of Caj++, the P-potential (ATP, ADP, AMP and Pi) and other metabolites, hormone contents and release. Third, it maintains a broad and well characterized stock of transformed islet cells, grows large batches of such cells or generates pseudo islets by embedding such cells into agarose beads for dynamic studies of metabolism and hormone release. Fourth and most important, it provides in depth consultation and helps develop strategies how to use the services of the core optimally or will attempt to modify available technologies to solve particular problems. The islet cell resource permits the broad application of approaches which are too labor intensive and technically too demanding to be maintained by the average single laboratory. The core offers training to those who have the resources and wish to establish approved procedures and technologies in their own laboratory. Costs are reduced through a large scale operation. The Core functions thus as a research support and educational unit for an active group of investigators with somewhat diverse interests, and fosters interdisciplinary islet research.

胰岛细胞生物学核心：主任- F. Matschinsky