MULTIPLE SITE OPTICAL RECORDING OF MEMBRANE POTENTIAL

膜电位的多位点光学记录

• 批准号: 3397157
• 负责人: BRIAN Matthew SALZBERG
• 金额: $ 27.6万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-12-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3397157
• 关键词: Anura; action potentials; axon; central nervous system; computer data analysis; dendrites; fluorescent dye /probe; laboratory mouse; membrane potentials; neuropeptides; neuropil; online computer; potentiometry; synapses; tissue /cell culture

Certain substances, when bound to the membranes of neurons, cardiac and skeletal muscle, glands, and other cells, behave as molecular indicators of membrane potential. The optical properties of these molecules vary linearly with potential and may, therefore, be used to monitor action potentials, synaptic potentials, or other changes in membrane voltage from a large number of sites at once, without the use of electrodes. The project will develop more sensitive probes, add to our understanding of the mechanisms by which they transduce voltage, and extend the technology associated with their use. The intention is to use these molecular voltmeters for optical recording of membrane potential from otherwise inaccessible regions of single neurons such as nerve terminals and dendritic arborizations, and from many sites simultaneously in small ensembles of neurons in tissue culture in order to study the spatial and temporal patterning of activity. First, a new high resolution computer based system for Multiple Site Optical Recording of Transmembrane Voltage (MSORTV) will be constructed, capable of monitoring changes in membrane potential from as many as 464 at once, and this apparatus will be used to study the properties of truly simple nervous systems - small ensembles of synaptically connected Aplysia central neurons maintained in culture - by optical recording of electrical activity from all of their components simultaneously. Second, this apparatus will be used to record membrane potential changes from fine processes of single neurons in order to determine the electrical properties, both active and passive, of neuronal structures which are not penetrable by electrodes and are frequently too far away, electrically, for their activity to be reflected in the somata. Finally, the project will exploit the laboratory's unique ability to monitor electrical activity in the nerve terminals of vertebrates to study the ion channels at the release sites of neuropeptides, and to combine absorption measurements of excitation at nerve terminals with photon correlation studies, using laser light scattering, in order better to understand how excitation is coupled to secretion, in vertebrate neurons and elsewhere.

某些物质，当与神经元、心脏和神经细胞的膜结合时 骨骼肌、腺体和其他细胞，充当分子指标 膜电位。 这些分子的光学性质各不相同 与潜力呈线性关系，因此可用于监测行动 电位、突触电位或膜电压的其他变化 一次检测大量部位，无需使用电极。 这 项目将开发更灵敏的探针，增加我们对 它们转换电压的机制并扩展了技术 与其使用相关。 目的是利用这些分子 用于光学记录膜电位的电压表 单个神经元的不可接近区域，例如神经末梢和 树突状树枝化，并且在小范围内同时来自许多位点 组织培养中的神经元集合，以研究空间和 活动的时间模式。 首先，一个新的基于高分辨率计算机的多站点系统 将构建跨膜电压光学记录（MSORTV）， 能够监测多达 464 个点的膜电位变化 一次，该设备将用于研究真正的属性 简单神经系统 - 突触连接的海兔的小集合 中枢神经元维持在培养物中 - 通过电学的光学记录 它们所有组件的活动同时进行。 其次，该装置将用于记录膜电位的变化 从单个神经元的精细过程中以确定电 神经元结构的主动和被动属性 可被电极穿透，并且通常距离电气太远，无法 他们的活动反映在体细胞上。 最后，该项目将利用实验室的独特能力 监测脊椎动物神经末梢的电活动以进行研究 神经肽释放位点的离子通道，并结合 光子在神经末梢激发的吸收测量 相关性研究，使用激光光散射，以便更好地 了解脊椎动物神经元中的兴奋与分泌如何耦合 和其他地方。