METABOLISM OF CELLS IN MUSCLE AND NERVOUS SYSTEM

目标是帮助满足一个重要的需求，即了解 肌肉和神经中不同类型细胞的生化性质 系统。大脑的功能依赖于各种细胞的相互作用， 盛大地相互纠缠在一起。多种细胞类型的混合物和 功能状态因细胞而异的事实，以及 每时每刻，意味着宏观化学方法仅限于 有用处。 肌肉也是一种亲密的细胞混合物，它们在 化学成分。彼此相邻的肌肉纤维也有同样的变化 在特定的酶之间的比例是50倍，以及差异 在肌肉疾病中可能被夸大。因此，这里也是宏观的 研究还不充分。 为了应对这种情况，可以使用一种分析方法，该方法具有 几乎无限的灵敏度，这使得测量成为可能 单个神经细胞体中的几乎任何酶或代谢物，在 大神经元，在其他大小相似的神经元结构中，以及在小神经元中 单个肌肉纤维的碎片。 本项目的目的是利用这种分析能力在 对肌肉和神经系统的研究。在某些情况下，单个神经元 或其部分被检查；在其他情况下，小组细胞被 学习。在肌肉的情况下，技术是如此的相同 单个纤维可以分析出大量的酶、代谢物和 辅因。

The objective is to help satisfy an important need, the need to know the biochemical nature of the different kinds of cells in muscle and nervous system. Brain function depends on the interplay of diverse kinds of cells, grandly entangled with each other. The admixture of the many cell types and the fact that the functional state differs from cell to cell, and from moment to moment, means that macroscopic chemical approaches are limited in usefulness. Muscle is also an intimate mixture of cells which differ greatly in chemical composition. Muscle fibers lying next to each other vary as much as 50-fold in the ratios between particular enzymes, and the differences can be exaggerated in muscle disease. Therefore, here also macroscopic studies are inadequate. To attack this situation, an analytical methodology is available which has virtually unlimited sensitivity and which makes it possible to measure nearly any enzyme or metabolite in single nerve cell bodies, in nuclei of large neurons, in other neuronal structures of similar size, and in small pieces of individual muscle fibers. It is the purpose of this project to exploit this analytical capability in the study of muscle and the nervous system. In some cases, single neurons or portions thereof are examined; in other cases, small groups of cells are studied. In the case of muscle, the technique is such that the same individual fibers can be analyzed for a great many enzymes, metabolites and cofactors.