MEASUREMENT OF RAPID CHANGE IN REGIONAL BRAIN METABOLISM

该提议是进一步测试、改进和利用开发的过程 在这个使用2-脱氧葡萄糖(2DG)来测量短期 大脑不同部位葡萄糖代谢率的变化。这个 著名的索科洛夫2DG法测量葡萄糖代谢率 ~(14)C-2DG-6-磷酸(DG6P)的放射自显影 在45分钟的时间段内。这一漫长的时期在很大程度上需要 消散2DG，这在无线电签名中是无法区分的 从它的磷酸盐代谢物。因为在大脑中有许多有趣的事件 在短得多的时间范围内发生，45分钟的延迟表示 对原有的2DG方法有明显的局限性。 新的方法使用非放射性的2DG和替代 放射自显影，直接酶法测定DG6P、2DG和葡萄糖 在大脑感兴趣的区域。短到一分钟的时间都很实用 对小到大脑膜的脑区的分析也是可行的。 这三种化合物不需要物理分离。化验结果 是基于区分DG6P和G6P的酶方法，并且 根据需要，能够进行几乎无限的放大。

The proposal is to further test, refine, and exploit a procedure developed in this laboratory which uses 2-deoxyglucose (2DG) to measure short-term changes in glucose metabolic rates in different parts of the brain. The well known 2DG method of Sokoloff measures glucose metabolic rate radioautographically by the accumulation of 14C-2DG-6-phosphate (DG6P) during a 45 min time period. This long period is required largely to dissipate the 2DG, which would be indistinguishable in the radioautograph from its phosphate metabolite. Because many events of interest in brain occur within a much shorter time frame, the 45 min delay represents a distinct limitation on the original 2DG method. The new method uses non-radioactive 2DG and substitutes for radioautography, the direct enzymatic measurement of DG6P, 2DG, and glucose in the brain areas of interest. Times as short as one minute are practical and analyses of brain areas as small as large perikarya are feasible. Physical separations of the three compounds are not required. The assays are based on enzymatic methods which distinguish DG6P from G6P, and which are capable of virtually unlimited amplification, according to need.