MEASUREMENT OF RAPID CHANGE IN REGIONAL BRAIN METABOLISM

区域脑代谢快速变化的测量

• 批准号: 3409551
• 负责人: OLIVER H LOWRY
• 金额: $ 12.74万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3409551
• 关键词: adenosine triphosphate; anticonvulsants; autoradiography; brain metabolism; carbon; cerebral ischemia /hypoxia; combination chemotherapy; convulsants; creatine phosphate; deoxyglucose; diagnosis design /evaluation; generalized seizures; glucose 6 phosphate; glucose metabolism; laboratory mouse; lactates; neurochemistry; radionuclides; radiotracer; sedative /hypnotic

The proposal is to further test, refine, and exploit a procedure developed in this laboratory which uses 2-deoxyglucose (2DG) to measure short-term changes in glucose metabolic rates in different parts of the brain. The well known 2DG method of Sokoloff measures glucose metabolic rate radioautographically by the accumulation of 14C-2DG-6-phosphate (DG6P) during a 45 min time period. This long period is required largely to dissipate the 2DG, which would be indistinguishable in the radioautograph from its phosphate metabolite. Because many events of interest in brain occur within a much shorter time frame, the 45 min delay represents a distinct limitation on the original 2DG method. The new method uses non-radioactive 2DG and substitutes for radioautography, the direct enzymatic measurement of DG6P, 2DG, and glucose in the brain areas of interest. Times as short as one minute are practical and analyses of brain areas as small as large perikarya are feasible. Physical separations of the three compounds are not required. The assays are based on enzymatic methods which distinguish DG6P from G6P, and which are capable of virtually unlimited amplification, according to need.

该提案旨在进一步测试、完善和利用开发的程序 在这个实验室中，使用 2-脱氧葡萄糖 (2DG) 来测量短期 大脑不同部位的葡萄糖代谢率发生变化。这 Sokoloff 著名的 2DG 方法测量葡萄糖代谢率 通过 14C-2DG-6-磷酸 (DG6P) 的积累进行放射自显影 在 45 分钟的时间段内。这段漫长的时间主要是为了 消散 2DG，这在放射自显影中是无法区分的 来自其磷酸盐代谢物。因为大脑中许多感兴趣的事件 发生在更短的时间内，45 分钟的延迟代表 原始 2DG 方法的明显局限性。 新方法使用非放射性 2DG 并替代 放射自显影，DG6P、2DG 和葡萄糖的直接酶法测量 在感兴趣的大脑区域。时间短至一分钟即可实用 对小到大核周的大脑区域进行分析都是可行的。 不需要对三种化合物进行物理分离。化验 基于区分 DG6P 和 G6P 的酶法，并且 根据需要，几乎可以无限放大。

项目成果

Glucose metabolism assessed with 2-deoxyglucose and the effect of glutamate in subdivisions of rat hippocampal slices.

用 2-脱氧葡萄糖评估葡萄糖代谢和谷氨酸对大鼠海马切片细分的影响。

• DOI: 10.1111/j.1471-4159.1992.tb11027.x
• 发表时间: 1992
• 期刊: Journal of neurochemistry
• 影响因子: 4.7
• 作者: McDougalJr,DB;Carter,JG;Pusateri,ME;Manchester,JK;Lowry,OH
• 通讯作者: Lowry,OH

A refinement of the Akabayashi-Saito-Kato modification of the enzymatic methods for 2-deoxyglucose and 2-deoxyglucose 6-phosphate.

2-脱氧葡萄糖和 2-脱氧葡萄糖 6-磷酸酶促方法的 Akabayashi-Saito-Kato 改进的改进。

• DOI: 10.1006/abio.1993.1130
• 发表时间: 1993
• 期刊: Analytical biochemistry
• 影响因子: 2.9
• 作者: Chi,MM;Manchester,JK;Carter,JG;Pusateri,ME;McDougal,DB;Lowry,OH
• 通讯作者: Lowry,OH

Glutamate and potassium stimulation of hippocampal slices metabolizing glucose or glucose and pyruvate.

谷氨酸和钾刺激海马切片代谢葡萄糖或葡萄糖和丙酮酸。

• DOI: 10.1016/s0006-8993(97)00129-7
• 发表时间: 1997
• 期刊: Brain research
• 影响因子: 2.9
• 作者: McDougalJr,DB;Cowsette,BR;Pusateri,ME;Carter,JG;Manchester,JK;Chi,MM;Lowry,OH
• 通讯作者: Lowry,OH

Use of nonradioactive 2-deoxyglucose to study compartmentation of brain glucose metabolism and rapid regional changes in rate.

• DOI: 10.1073/pnas.87.4.1357
• 发表时间: 1990-02
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: D. McDougal;J. Ferrendelli;V. Yip;M. E. Pusateri;J. Carter;M. Chi;B. Norris;J. Manchester;O. H. Lowry

Changes in ATP, phosphocreatine, and 16 metabolites in muscle stimulated for up to 96 hours.

刺激长达 96 小时的肌肉中 ATP、磷酸肌酸和 16 种代谢物的变化。

• DOI: 10.1152/ajpcell.1996.271.4.c1167
• 发表时间: 1996
• 期刊: The American journal of physiology.
• 作者: Salmons,S;Jarvis,JC;Mayne,CN;Chi,MM;Manchester,JK;McDougalJr,DB;Lowry,OH
• 通讯作者: Lowry,OH