MEASUREMENT OF RAPID CHANGE IN REGIONAL BRAIN METABOLISM
MEASUREMENT OF RAPID CHANGE IN REGIONAL BRAIN METABOLISM
批准号:
3409550
负责人:
OLIVER H LOWRY
金额:
$12.25万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1987
资助国家:
美国
项目状态:
已结题
起止时间:
1987-04-01 至 1993-03-31
关键词:
adenosine triphosphate anticonvulsants autoradiography brain metabolism cerebral ischemia /hypoxia combination chemotherapy convulsants creatine phosphate deoxyglucose diagnosis design /evaluation generalized seizures glucose 6 phosphate glucose metabolism laboratory mouse lactates neurochemistry radiotracer sedative /hypnotic
中文摘要
该提议是进一步测试、改进和利用开发的过程
在这个使用2-脱氧葡萄糖(2DG)来测量短期
大脑不同部位葡萄糖代谢率的变化。这个
著名的索科洛夫2DG法测量葡萄糖代谢率
~(14)C-2DG-6-磷酸(DG6P)的放射自显影
在45分钟的时间段内。这一漫长的时期在很大程度上需要
消散2DG,这在无线电签名中是无法区分的
从它的磷酸盐代谢物。因为在大脑中有许多有趣的事件
在短得多的时间范围内发生,45分钟的延迟表示
对原有的2DG方法有明显的局限性。
新的方法使用非放射性的2DG和替代
放射自显影,直接酶法测定DG6P、2DG和葡萄糖
在大脑感兴趣的区域。短到一分钟的时间都很实用
对小到大脑膜的脑区的分析也是可行的。
这三种化合物不需要物理分离。化验结果
是基于区分DG6P和G6P的酶方法,并且
根据需要,能够进行几乎无限的放大。
英文摘要
The proposal is to further test, refine, and exploit a procedure developed
in this laboratory which uses 2-deoxyglucose (2DG) to measure short-term
changes in glucose metabolic rates in different parts of the brain. The
well known 2DG method of Sokoloff measures glucose metabolic rate
radioautographically by the accumulation of 14C-2DG-6-phosphate (DG6P)
during a 45 min time period. This long period is required largely to
dissipate the 2DG, which would be indistinguishable in the radioautograph
from its phosphate metabolite. Because many events of interest in brain
occur within a much shorter time frame, the 45 min delay represents a
distinct limitation on the original 2DG method.
The new method uses non-radioactive 2DG and substitutes for
radioautography, the direct enzymatic measurement of DG6P, 2DG, and glucose
in the brain areas of interest. Times as short as one minute are practical
and analyses of brain areas as small as large perikarya are feasible.
Physical separations of the three compounds are not required. The assays
are based on enzymatic methods which distinguish DG6P from G6P, and which
are capable of virtually unlimited amplification, according to need.
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会议论文
MEASUREMENT OF RAPID CHANGE IN REGIONAL BRAIN METABOLISM
-
批准号:3409549
-
项目类别:
-
资助金额:$10.61万
-
财政年份:1987
-
负责人:OLIVER H LOWRY
-
依托单位:
MEASUREMENT OF RAPID CHANGE IN REGIONAL BRAIN METABOLISM
-
批准号:3409551
-
项目类别:
-
资助金额:$12.74万
-
财政年份:1987
-
负责人:OLIVER H LOWRY
-
依托单位:
MEASUREMENT OF RAPID CHANGE IN REGIONAL BRAIN METABOLISM
-
批准号:3409548
-
项目类别:
-
资助金额:$10.43万
-
财政年份:1987
-
负责人:OLIVER H LOWRY
-
依托单位:
MEASUREMENT OF RAPID CHANGE IN REGIONAL BRAIN METABOLISM
-
批准号:3409546
-
项目类别:
-
资助金额:$11.61万
-
财政年份:1987
-
负责人:OLIVER H LOWRY
-
依托单位:
MEASUREMENT OF RAPID CHANGE IN REGIONAL BRAIN METABOLISM
-
批准号:3409545
-
项目类别:
-
资助金额:$10.85万
-
财政年份:1987
-
负责人:OLIVER H LOWRY
-
依托单位:
MOLECULAR, BIOCHEMICAL AND PHYSIOLOGICAL PHARMACOLOGY
-
批准号:3537915
-
项目类别:
-
资助金额:$5.57万
-
财政年份:1981
-
负责人:OLIVER H LOWRY
-
依托单位:
MOLECULAR, BIOCHEMICAL, AND PHYSIOLOGICAL PHARMACOLOGY
-
批准号:3537916
-
项目类别:
-
资助金额:$13.57万
-
财政年份:1981
-
负责人:OLIVER H LOWRY
-
依托单位:
MOLECULAR, BIOCHEMICAL AND PHYSIOLOGICAL PHARMACOLOGY
-
批准号:3537912
-
项目类别:
-
资助金额:$3.73万
-
财政年份:1981
-
负责人:OLIVER H LOWRY
-
依托单位:
MOLECULAR, BIOCHEMICAL AND PHYSIOLOGICAL PHARMACOLOGY
-
批准号:3537914
-
项目类别:
-
资助金额:$9.16万
-
财政年份:1981
-
负责人:OLIVER H LOWRY
-
依托单位:
NEUROPHARMACOLOGY RESEARCH TRAINING
-
批准号:3543658
-
项目类别:
-
资助金额:$11.1万
-
财政年份:1978
-
负责人:OLIVER H LOWRY
-
依托单位:
NEUROPHARMACOLOGY RESEARCH
-
批准号:3543662
-
项目类别:
-
资助金额:$16.71万
-
财政年份:1978
-
负责人:OLIVER H LOWRY
-
依托单位:
NEUROPHARMACOLOGY RESEARCH TRAINING
-
批准号:3543656
-
项目类别:
-
资助金额:$9.96万
-
财政年份:1978
-
负责人:OLIVER H LOWRY
-
依托单位:
NEUROPHARMACOLOGY RESEARCH TRAINING
-
批准号:3543659
-
项目类别:
-
资助金额:$11.0万
-
财政年份:1978
-
负责人:OLIVER H LOWRY
-
依托单位:
NEUROPHARMACOLOGY RESEARCH
-
批准号:3543660
-
项目类别:
-
资助金额:$10.67万
-
财政年份:1978
-
负责人:OLIVER H LOWRY
-
依托单位:
NEUROPHARMACOLOGY RESEARCH TRAINING
-
批准号:3543663
-
项目类别:
-
资助金额:$19.67万
-
财政年份:1978
-
负责人:OLIVER H LOWRY
-
依托单位:
NEUROPHARMACOLOGY RESEARCH
-
批准号:3543661
-
项目类别:
-
资助金额:$18.29万
-
财政年份:1978
-
负责人:OLIVER H LOWRY
-
依托单位:
METABOLISM OF CELLS IN MUSCLE AND NERVOUS SYSTEM
-
批准号:3393846
-
项目类别:
-
资助金额:$10.65万
-
财政年份:1974
-
负责人:OLIVER H LOWRY
-
依托单位:
METABOLISM OF CELLS IN MUSCLE AND NERVOUS SYSTEM
-
批准号:2260753
-
项目类别:
-
资助金额:$14.89万
-
财政年份:1974
-
负责人:OLIVER H LOWRY
-
依托单位:
METABOLISM OF CELLS IN MUSCLE AND NERVOUS SYSTEM
-
批准号:3393851
-
项目类别:
-
资助金额:$14.56万
-
财政年份:1974
-
负责人:OLIVER H LOWRY
-
依托单位:
METABOLISM OF CELLS IN MUSCLE AND NERVOUS SYSTEM
-
批准号:3393850
-
项目类别:
-
资助金额:$14.0万
-
财政年份:1974
-
负责人:OLIVER H LOWRY
-
依托单位:
海外基金