NEURONAL CONNECTIONS AND RESPONSES IN CINGULATE CORTEX

这项调查的目标是评估内在组织， 扣带回神经元的受体结合和反应特性， 边缘系统的主要组成部分之一。内在组织 将在兔体内用高尔基体和变性技术进行评估 大脑。快速高尔基材料将提供有关类型的信息 神经元及其轴突在皮质内的分布，特别是在 这些细胞是边缘皮质所特有的，包括外倾和 梭形金字塔。神经毒素鹅膏菌酸将被用来评估 较长内在连接的密度和范围。将会进行消融 在浅层或深层用这种毒素，然后光和 电子显微镜技术用来确定大多数神经元的位置 在特定的层中终止。M胆碱能受体 通过消融来定位于大鼠特定类型的神经元 使用鹅膏酸或乙酰胆碱芥末，然后评估 用体外试验测定受体密度的变化。这个化验 采用冷冻机切开扣带回皮质，然后孵育 不可逆毒毒碱配体H3-丙基苯基胆碱的截面 芥末。还建议进行彻底的超微结构分析，以便 准确地评估是什么形态变化导致了 刺激性毒素。将在眼睛期间评估29d区的神经元反应 动静。急性兔准备的细胞外录音将会 与红外线监测眼睛位置相结合 安装在隐形眼镜上的发光二极管。它们之间的位相关系 神经元放电与眼的方向、时程和波幅 将对行动进行评估。这些反应的连接基础 将通过丘脑消融和/或皮质降温得到澄清 除了解剖道追踪技术外，还有其他技术。 这些项目将使我们更全面地了解 胆碱能系统，因此将有助于解释 患者扣带回皮质的受体结合模式 患有阿尔茨海默氏症。最后，在进行解读和处理之前 对运动障碍的静止性沉默是可以做到的，这将是必要的 了解扣带回皮质的感觉运动功能。

The goals of this investigation are to evaluate the intrinsic organization, receptor binding and response properties of neurons in cingulate cortex, one of the major components of the limbic system. Intrinsic organization will be assessed with Golgi and degeneration techniques in the rabbit brain. Rapid Golgi material will provide information about the types of neurons and the intracortical distribution of their axons, particularly for those cells which are unique to limbic cortex including extraverted and fusiform pyramids. The neurotoxin ibotenic acid will be used to assess the density and extent of longer intrinsic connections. Ablations will be made in the superficial or deep layers with this toxin and then light and electron microscopic techniques used to determine where most of the neurons in a particular layer terminate. Muscarinic acetylcholine receptors will be localized to specific types of neurons in the rat by making ablations with ibotenic acid or acetylethycholine mustard followed by evaluation of changes in the density of receptors using an in vitro assay. This assay employs cryostat sectioning of cingulate cortex followed by incubation of the sections in the irreversible muscarinic ligand H3-propylbenzilylcholine mustard. A thorough ultrastructural analysis is also proposed in order to assess exactly what morphological changes underlie the action of excitotoxins. Neuronal responses will be evaluated in area 29d during eye movements. Extracellular recordings from an acute rabbit preparation will be made in conjunction with monitoring eye position with an infrared light emitting diode mounted on a contact lens. The phase relationships between neuronal discharges and the direction duration and amplitude of eye movements will be evaluated. The connectional basis of these responses will be clarified using thalamic ablation and/or cortical cooling techniques in addition to an anatomical tract tracing technique. These projects will lead to a more complete understanding of the cholinergic system and as such will help to interpret alterations in receptor binding patterns that occur in the cingulate cortices of patients with Alzheimer's disease. Finally, before an interpretation and treatment of the movement disorder akinetic mutism can be made, it will be necessary to understand the sensorimotor functions of cingulate cortex.