NEURAL CONTROL OF MAGNOCELLULAR NEUROENDOCRINE CELLS

巨细胞神经内分泌细胞的神经控制

• 批准号: 3397242
• 负责人: GLENN I HATTON
• 金额: $ 8.39万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-01-01 至 1986-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3397242
• 关键词: afferent nerve; electrophysiology; histochemistry /cytochemistry; immunochemistry; membrane structure; neural information processing; neural inhibition; neural initiation; neural transmission; neuroanatomy; neuroendocrine system; oxytocin; synapses; vasopressins

The long-range goal of our research is to understand the mechanisms involved in the control of hormone secretion from the magnocellular neuroendocrine cells (MNCs) whose cell bodies reside predominantly in the supraoptic (SON) and paraventricular (PVN) nuclei of the hypothalamus. Since the patterns of electrical activity are dominant factors in the process of neurosecretion by these cells, our immediate goals for the research proposed here are to investigate some of the factors which may influence or determine their activity patterns. In particular we wish to focus on investigating membrane properties, synaptic inputs and non-synaptic interactions, all of which probably play roles in controlling or modulating the process of neurosecretion in this system. In order to accomplish these objectives, we will record intracellular from PVN and perifornical neurons and from both neurons and glial cells in the area of the SON in slices of rat hypothalamus. In some instances the electrodes will be filled with the fluorescent dye, Lucifer Yellow (LY), and in others with horseradish peroxidase (HRP) in order to identify the recorded cell and trace its processes. Cells marked with LY will undergo either subsequent immunocytochemical analysis of hormone content (oxytocin, vasopressin or enkephalin) or electron microscopic (EM) analysis to demonstrate gap junctions after rendering the LY electron dense. HRP-injected cells and their processes will be analyzed at both the light and EM levels in order to determine the collateral branching and axonal terminations of these neurons. Studies of putative transmitters will also be carried out. Thus, we will be able to determine the physiological, morphological and hormonal characteristics of cells in this brain area, as well as to learn more about the local circuitry in which these cell groups apparently participate.

我们研究的长期目标是了解这些机制 参与控制巨噬细胞的激素分泌 神经内分泌细胞(MNC)，其胞体主要位于 下丘脑视上核(SON)和室旁核(PVN)。 由于电活动的模式是导致 这些细胞的神经分泌过程，我们的直接目标是 这里提出的研究是为了调查一些可能 影响或决定他们的活动模式。我们尤其希望 重点研究细胞膜特性，突触输入和 非突触相互作用，所有这些都可能在控制 或者调节这个系统中的神经分泌过程。 为了实现这些目标，我们将记录来自 室旁核和穹隆周围神经元以及神经元和神经胶质细胞在 大鼠下丘脑切片中SON的面积。在某些情况下， 电极上将填充荧光染料--荧光黄(LY)， 另一些则用辣根过氧化物酶(HRP)来鉴定 记录细胞并追踪其过程。标记了LY的单元格将经历 随后的激素含量的免疫细胞化学分析(催产素， 加压素或脑啡肽)或电子显微镜(EM)分析 演示呈现密集的LY电子后的缝隙结。 HRP注射的细胞及其过程将在两种光下进行分析 和EM水平，以确定侧支和轴突 这些神经元的终末。对假定的发射器的研究也将 被执行。因此，我们将能够确定生理上的， 这一脑区细胞的形态和激素特征，如 以及了解更多关于这些电池组所在的本地电路的信息 显然是参与其中。