CA2+,H+, AND NEUROSECRETION IN BRAIN NERVE TERMINALS

这项研究的目的是了解一些基本的 哺乳动物突触前神经末梢的特性。 夹断神经 将使用从大鼠脑中分离的末端（突触体），因为它们 保留了许多完整的功能，代谢和形态学特性 神经元组织，并且非常适合涉及 放射性示踪剂通量、光学和生物化学技术。 研究者 将：1）检查突触前Ca通道的特性 参与神经分泌-渗透性和选择性，以及 失活; 2）确定阳离子如何渗透，并选择性地 阻断，Ca通道影响Ca的封存和排出以及传递 释放; 3）测量神经末梢的细胞内pH值，研究它是如何释放的。 调节，以及它如何与Ca通道活性相关， 神经分泌; 4）开发和利用用于装载Ca指示剂的方法 进入神经末梢，使钙代谢及其关系， 可以研究神经末梢的活动。 本项目将提供 更好地理解控制细胞的基本机制， 细胞内钙和pH值在脑神经末梢，以及如何调节 影响大脑神经递质的释放。

The goal of the proposed research is to understand some of the fundamental properties of mammalian presynaptic nerve terminals. Pinched-off nerve endings (synaptosomes) isolated from rat brain will be used, because they retain many functional, metabolic, and morphological properties of intact neuronal tissue, and are well-suited for studies that involve radiotracer-flux, optical, and biochemical techniques. The Investigator will: 1) examine the characteristics of the presynaptic Ca channels involved in neurosecretion - permeability and selectivity, and mechanism of inactivation; 2) determine how cations that permeate, and selectively block, Ca channels influence Ca sequestration and extrusion and transmitter release; 3) measure intracellular pH in the nerve terminals, study how it is regulated, and how it is related to Ca channel activity and neurosecretion; 4) develop and utilize methods for loading Ca-indicators into nerve terminals, so that Ca metabolism and its relation to the activity of nerve terminals can be investigated. This project will provide a better understanding of the basic cellular mechanisms that control intracellular Ca and pH in brain nerve endings, and of how this regulation affects the relase of brain neurotransmitters.