CA2+,H+, AND NEUROSECRETION IN BRAIN NERVE TERMINALS
CA2+,H+, AND NEUROSECRETION IN BRAIN NERVE TERMINALS
批准号:
3400796
负责人:
OLAF S. ANDERSEN
金额:
$7.08万
依托单位国家:
美国
项目类别:
财政年份:
1983
资助国家:
美国
项目状态:
已结题
起止时间:
1983-12-01 至 1987-12-31
中文摘要
这项研究的目的是了解一些基本的
哺乳动物突触前神经末梢的特性。 夹断神经
将使用从大鼠脑中分离的末端(突触体),因为它们
保留了许多完整的功能,代谢和形态学特性
神经元组织,并且非常适合涉及
放射性示踪剂通量、光学和生物化学技术。 研究者
将:1)检查突触前Ca通道的特性
参与神经分泌-渗透性和选择性,以及
失活; 2)确定阳离子如何渗透,并选择性地
阻断,Ca通道影响Ca的封存和排出以及传递
释放; 3)测量神经末梢的细胞内pH值,研究它是如何释放的。
调节,以及它如何与Ca通道活性相关,
神经分泌; 4)开发和利用用于装载Ca指示剂的方法
进入神经末梢,使钙代谢及其关系,
可以研究神经末梢的活动。 本项目将提供
更好地理解控制细胞的基本机制,
细胞内钙和pH值在脑神经末梢,以及如何调节
影响大脑神经递质的释放。
英文摘要
The goal of the proposed research is to understand some of the fundamental
properties of mammalian presynaptic nerve terminals. Pinched-off nerve
endings (synaptosomes) isolated from rat brain will be used, because they
retain many functional, metabolic, and morphological properties of intact
neuronal tissue, and are well-suited for studies that involve
radiotracer-flux, optical, and biochemical techniques. The Investigator
will: 1) examine the characteristics of the presynaptic Ca channels
involved in neurosecretion - permeability and selectivity, and mechanism of
inactivation; 2) determine how cations that permeate, and selectively
block, Ca channels influence Ca sequestration and extrusion and transmitter
release; 3) measure intracellular pH in the nerve terminals, study how it
is regulated, and how it is related to Ca channel activity and
neurosecretion; 4) develop and utilize methods for loading Ca-indicators
into nerve terminals, so that Ca metabolism and its relation to the
activity of nerve terminals can be investigated. This project will provide
a better understanding of the basic cellular mechanisms that control
intracellular Ca and pH in brain nerve endings, and of how this regulation
affects the relase of brain neurotransmitters.
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Measurement of the cytosolic sodium ion concentration in rat brain synaptosomes by a fluorescence method.
荧光法测定大鼠脑突触体中胞质钠离子浓度。
DOI:
10.1016/0005-2736(88)90198-8
发表时间:
1988
期刊:
Biochimica et biophysica acta
影响因子:
--
作者:
[Kongsamut,S, Nachshen,DA]
通讯作者:
Nachshen,DA
'Slow' K+-stimulated Ca2+ influx is mediated by Na+-Ca2+ exchange: a pharmacological study.
“缓慢”K 刺激的 Ca2 流入是由 Na -Ca2 交换介导的:一项药理学研究。
DOI:
10.1016/0005-2736(89)90249-6
发表时间:
1989
期刊:
Biochimica et biophysica acta
影响因子:
--
作者:
[Nachshen,DA, Kongsamut,S]
通讯作者:
Kongsamut,S
DOI:
10.1085/jgp.91.2.305
发表时间:
1988-02
期刊:
The Journal of general physiology
影响因子:
--
作者:
[Drapeau P, Nachshen DA]
通讯作者:
Nachshen DA
Regulation of cytosolic calcium concentration in presynaptic nerve endings isolated from rat brain.
大鼠脑突触前神经末梢胞质钙浓度的调节。
DOI:
10.1113/jphysiol.1985.sp015697
发表时间:
1985
期刊:
The Journal of physiology
影响因子:
--
作者:
[Nachshen,DA]
通讯作者:
Nachshen,DA
The regulation of cytosolic pH in isolated presynaptic nerve terminals from rat brain.
大鼠脑中分离的突触前神经末梢细胞质 pH 值的调节。
DOI:
10.1085/jgp.91.2.289
发表时间:
1988
期刊:
The Journal of general physiology
影响因子:
--
作者:
[Nachshen,DA, Drapeau,P]
通讯作者:
Drapeau,P
J. NRSA Training Core
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项目类别:
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资助金额:$66.83万
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财政年份:2017
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依托单位:
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批准号:10632544
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Putting Molecular Dynamics to the Test: Ion Permeation
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Putting Molecular Dynamics to the Test: Ion Permeation
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批准号:6973714
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项目类别:
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资助金额:$41.08万
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财政年份:2005
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负责人:OLAF S. ANDERSEN
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依托单位:
Putting Molecular Dynamics to the Test: Ion Permeation
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批准号:7462411
-
项目类别:
-
资助金额:$38.04万
-
财政年份:2005
-
负责人:OLAF S. ANDERSEN
-
依托单位:
Putting Molecular Dynamics to the Test: Ion Permeation
-
批准号:7263134
-
项目类别:
-
资助金额:$38.06万
-
财政年份:2005
-
负责人:OLAF S. ANDERSEN
-
依托单位:
VOLTAGE DEPENDENT SODIUM CHANNEL--PLANAR LIPID BILAYER
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批准号:3297367
-
项目类别:
-
资助金额:$15.51万
-
财政年份:1988
-
负责人:OLAF S. ANDERSEN
-
依托单位:
VOLTAGE DEPENDENT SODIUM CHANNELS IN PLANAR LIPID BILAYE
-
批准号:3297362
-
项目类别:
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资助金额:$14.65万
-
财政年份:1988
-
负责人:OLAF S. ANDERSEN
-
依托单位:
VOLTAGE DEPENDENT SODIUM CHANNEL--PLANAR LIPID BILAYER
-
批准号:3297364
-
项目类别:
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资助金额:$13.64万
-
财政年份:1988
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负责人:OLAF S. ANDERSEN
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依托单位:
VOLTAGE DEPENDENT SODIUM CHANNEL--PLANAR LIPID BILAYER
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-
项目类别:
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资助金额:$13.91万
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依托单位:
VOLTAGE DEPENDENT SODIUM CHANNEL--PLANAR LIPID BILAYER
-
批准号:3297366
-
项目类别:
-
资助金额:$14.9万
-
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-
负责人:OLAF S. ANDERSEN
-
依托单位:
Weill Cornell/Rockefeller/Sloan-Kettering MST Program
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资助金额:$192.1万
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财政年份:1979
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负责人:OLAF S. ANDERSEN
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批准号:9103128
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项目类别:
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资助金额:$224.87万
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财政年份:1979
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负责人:OLAF S. ANDERSEN
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依托单位:
CORNELL/ROCKEFELLER/SLOAN-KETTERING MST PROGRAM
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批准号:6350991
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负责人:OLAF S. ANDERSEN
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依托单位:
海外基金