CA2+,H+, AND NEUROSECRETION IN BRAIN NERVE TERMINALS

这项拟议的研究的目标是了解一些基本的 哺乳动物突触前神经末梢的特性。夹断的神经 将使用从大鼠脑中分离的终末(突触体)，因为它们 保留了许多完整的功能、代谢和形态特性 神经细胞组织，非常适合研究涉及 放射性示踪剂-助熔剂、光学和生化技术。《调查者》 将：1)检查突触前钙通道的特征 参与神经分泌--通透性和选择性，以及 失活；2)确定阳离子如何渗透，并选择性地 阻断、钙通道影响钙的固存、排出和递质 释放；3)测量神经末梢的细胞内pH，研究它是如何 受调控，以及它与钙通道活性和 神经分泌；4)开发和利用钙指示剂的负载方法 进入神经末梢，从而使钙代谢及其与 可以研究神经末梢的活动情况。该项目将提供 更好地理解控制细胞的基本机制 脑神经末梢的细胞内钙和pH，以及这种调节是如何 影响脑神经递质的释放。

The goal of the proposed research is to understand some of the fundamental properties of mammalian presynaptic nerve terminals. Pinched-off nerve endings (synaptosomes) isolated from rat brain will be used, because they retain many functional, metabolic, and morphological properties of intact neuronal tissue, and are well-suited for studies that involve radiotracer-flux, optical, and biochemical techniques. The Investigator will: 1) examine the characteristics of the presynaptic Ca channels involved in neurosecretion - permeability and selectivity, and mechanism of inactivation; 2) determine how cations that permeate, and selectively block, Ca channels influence Ca sequestration and extrusion and transmitter release; 3) measure intracellular pH in the nerve terminals, study how it is regulated, and how it is related to Ca channel activity and neurosecretion; 4) develop and utilize methods for loading Ca-indicators into nerve terminals, so that Ca metabolism and its relation to the activity of nerve terminals can be investigated. This project will provide a better understanding of the basic cellular mechanisms that control intracellular Ca and pH in brain nerve endings, and of how this regulation affects the relase of brain neurotransmitters.