NEURONAL ORGANIZATION OF THE BASOLATERAL AMYGDALA

这项拟议的研究是一项长期调查的继续， 神经元，神经化学，hodological，和突触组织的 哺乳动物杏仁核的外侧核和基底外侧核（L-BL）。 先前 这个实验室的研究提供了重要的新信息 关于L-BL的投射和递质免疫组织化学， 老鼠 拟议的调查将探讨其他hodological 和神经化学方面的L-BL在大鼠，并将启动类似 灵长类杏仁核同源核的研究。 大鼠研究 将：1）确定哪些细胞类型利用谷氨酸作为 神经递质; 2）确定是否神经肽血管活性肠 多肽（VIP）、生长抑素（SOM）和胆囊收缩素（CCK）是 在含有抑制性神经递质的神经元中发现 γ-氨基丁酸（GABA）; 3）检查 终止来自不同脑区的L-BL传入; 4）分析 通过免疫细胞化学技术鉴定神经元的突触学。 对灵长类动物的研究将：1）描述和分类不同类型的 L-BL神经元的形态学差异的基础上，Golgi染色 脑; 2）表征含有VIP、SOM、CCK、GABA和 谷氨酸; 3）确定神经肽是否与GABA共存， 神经元 用于实现这些目标的技术包括高尔基体 光镜和电镜免疫细胞化学技术 水平和PHA-L顺行追踪技术。 计算机辅助 将使用统计程序来关联被染色的神经元。 不同的技术。 杏仁核是参与学习的两个主要前脑区域之一， 显示了阿尔茨海默病的神经病理学改变。 也是 颞叶癫痫是癫痫最常见的形式， 通常与攻击性和其他精神病理性 变化 研究对象中待研究肽的杏仁体水平 在精神分裂症和精神分裂症的发展中， 这一地区的癫痫现象。 据了解， GABA相关的抗焦虑和抗惊厥作用 苯二氮卓类药物（安定和利多卡因）是L-BL。 信息 L-BL的连接和化学微电路应该有助于 了解其药理学、生理学和病理生理学。 未来 该实验室将继续对啮齿动物和灵长类动物进行研究， 探索L-BL神经化学突触学的重要方面， 超微结构水平

The proposed study is the continuation of a long-term investigation of the neuronal, neurochemical, hodological, and synaptic organization of the lateral and basolateral nuclei (L-BL) of the mammalian amygdala. Previous studies in this laboratory have provided important new information regarding the projections and transmitter immunohistochemistry of L-BL in the rat. The proposed investigation will explore additional hodological and neurochemical aspects of L-BL in the rat and will initiate similar studies of homologous nuclei in the primate amygdala. Studies in the rat will: 1) determine which cell types utilize glutamate as a neurotransmitter; 2) determine if the neuropeptides vasoactive intestinal polypeptide (VIP), and somatostatin (SOM) and cholecystokinin (CCK) are found in neurons that also contain the inhibitory neurotransmitter gamma-aminobutyric acid (GABA); 3) examine the precise pattern of termination of afferents to L-BL from various brain regions; 4) analyze the synaptology of neurons identified by immunocytochemical techniques. Studies in primates will: 1) describe and classify the different types of L-BL neurons on the basis of morphological differences in Golgi-stained brains; 2) characterize the cell types that contain VIP, SOM, CCK, GABA and glutamate; 3) determine if the neuropeptides coexist with GABA in some neurons. The techniques used to accomplish these aims include the Golgi technique, immunocytochemistry at the light and electron microscopic levels, and PHA-L anterograde tract tracing technique. Computer-assisted statistical procedures will be used to correlate neurons stained by different techniques. The amygdala is one of two major forebrain areas involved in learning and shows neuropathological alterations in Alzheimer's disease. It is also involved in temporal lobe epilepsy, the most common form of epilepsy and a type frequently associated with aggression and other psychopathological changes. Amygdaloid levels of the peptides to be investigated in the proposed study are altered in schizophrenia and in the development of epileptic phenomena in this area. It is also known that the chief site of the anti-anxiety and anti-convulsant actions of the GABA-related benzodiazepine drugs (Valium and Librium) is L-BL. Information on the connections and chemical microcircuitry of L-BL should contribute to an understanding of its pharmacology, physiology and pathophysiology. Future studies of both rodents and primates in this laboratory will continue to explore important aspects of the neurochemical synaptology of L-BL at the ultrastructural level.