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CYTOLOGY AND CONNECTIONS OF THE AUDITORY SYSTEM

CYTOLOGY AND CONNECTIONS OF THE AUDITORY SYSTEM
听觉系统的细胞学和连接
批准号:
3402293
负责人:
JOE C ADAMS
金额:
$8.38万
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-07-01 至 1987-06-30

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中文摘要
翻译
延髓听神经的细胞学和细胞类型的联系 人们将通过各种技术来研究原子核。以下是单个单位 外侧区或周围细胞生理反应的记录 上等橄榄的猫,辣根过氧化物酶(HRP)会 离子漂浮在细胞外。逆行标记的细胞 所应用的HRP的运输将根据其位置和 尼氏染色图谱。如有必要,这将通过删除 HRP反应产物的含量。如果它还不是已知的,它的形态 将使用高尔基来研究如此识别的小区类别, 银还原、组织化学和免疫组织化学技术。相同 细胞学研究将对外侧上叶内的细胞进行 奥利芙。黄曲霉属植物细胞学及细胞类联系的研究 CAT将为与人类材料进行比较奠定基础 将进行类似的细胞学和免疫组织化学研究。通过 建立单元类之间的联系并刻画它们 在猫的细胞学中,细胞类别的连接同样 可以推断出人类的细胞学特征。了解 这两个物种的听觉脑干的组织结构是由 高级。
英文摘要
The cytology and connection of cell classes in the medullary auditory nuclei will be studied by a variety of techniques. Following single unit recordings of physiological responses of cells in or around the lateral superior olive of the cat, horseradish peroxidase (HRP) will be iontophoresed extracellularly. The cells that are labelled by retrograde transport of the applied HRP will be identified by their locations and Nissl staining pattern. If necessary this will be accomplished by removal of the HRP reaction product. If it is not already known, the morphology of the cell classes that are so identified will be studied using Golgi, reduced silver, histochemical and immunohistochemical techniques. The same cytological studies will be done on cells within the lateral superior olive. Studies of the cytology and connections of the cell classes in the cat will provide the foundation for comparisons with human material in which similar cytological and immunohistochemical studies will be made. By establishing the connections of cell classes and characterizing them cytologically in the cat, the connections of cell classes that are likewise cytologically characterized in the human can be inferred. Understanding of the organization of the auditory brainstem in both species is therby advanced.
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