HISTOLOGY/SURGERY CORE

组织学/外科核心提供智力和物理环境，中心 研究人员，他们的实习生和支持人员可以学习和执行各种外科和组织学 程序。核心将1)目标1A、2A：维护现有的动物外科共享设施和 组织学处理，2)目标1B、2B：提供外科技术方面的技术专长和培训(两者 急性和慢性)和组织学技术，用于外周和中枢听觉的组织处理 光学和电子显微镜层次的结构，3)目标2D：继续研究改进和 改进组织学和外科手术方法，以及4)目标1E：协助饲养老鼠维持 以及繁殖突变品系。 一些复杂的外科技术，如麻醉猫的神经生理准备 研究将作为一项技术服务例行提供(AIMS 1C、1D)。一些组织学处理将是 作为技术服务执行，但仅当任务复杂性和有限的项目范围使其效率低下时才执行 对相关小组进行培训(目标2C)。设施维护将包括1)订购用品，2)准备 库存解决方案，3)设备维修，4)遵守当地和联邦法规。所有研究 中心的团队将受益于不复制这些基本服务而节省的时间。 所有调查人员还将受益于核心所代表的经验和专家咨询。 首先，通过将培训新学员和新员工的任务转嫁给核心人员，将节省大量时间 调查员专用的支持人员。第二，核心专业知识将提高所有参与者的研究能力1) 促进新技术的结合和2)允许他们使用各种技术，每种技术 在阶段性的基础上，而不必在自己的团队中保持所有必要的技能。 CORE的工作人员是一名经验丰富的个人，在所有相关领域都有证明的专业知识，包括 动物外科、免疫组织化学以及广泛的光和光染色技术 外周和中枢听觉结构的电子显微镜评估。

The Histology/Surgery Core provides the intellectual and physical environment in which Center Investigators, their trainees and support staff can learn and perform a variety of surgical and histological procedures. The Core will 1) Aims 1A, 2A: maintain existing shared facilities for animal surgery and histological processing, 2) Aims 1B, 2B: provide technical expertise and training in surgical techniques (both acute and chronic) and histological techniques for tissue processing of both peripheral and central auditory structures at the light- and electron-microscopic levels, 3) Aim 2D: continue to research improvements and refinements to histological and surgical methods, and 4) Aim 1E: assist with mouse husbandry in maintaining and propagating mutant lines. Some complex surgical techniques, such as preparation of the anesthetized cat for neurophysiological study, will be routinely offered as a technical service (Aims 1C, 1D). Some histological processing will be performed as a technical service, but only when task complexity and limited project scope make it inefficient to train the relevant group (Aim 2C). Facility maintenance will include 1) ordering of supplies, 2) preparation of stock solutions, 3) equipment repair, and 4) compliance with local and federal regulations. All research groups in the Center will benefit by the time saved in not duplicating these basic services. All investigators will also benefit from the repository of experience and expert advice the Core represents. First, significant time will be saved by offloading to Core personnel the task of training new students and new investigator-specific support staff. Second, Core expertise will enhance the research of all participants by 1) facilitating the incorporation of new techniques and 2) allowing them to use a wide range of techniques, each on a phasic basis, without having to maintain all the requisite skills within their own group. Core is staffed by one experienced individual with demonstrated expertise in all relevant areas, including animal surgery, immunohistpchemistry and a wide range of embedding and staining techniques for light- and electron-microscopic evaluation of peripheral and central auditory structures.