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CYTOLOGY AND CONNECTIONS OF THE AUDITORY SYSTEM

CYTOLOGY AND CONNECTIONS OF THE AUDITORY SYSTEM
听觉系统的细胞学和连接
批准号:
3402291
负责人:
JOE C ADAMS
金额:
$13.86万
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-07-01 至 1992-06-30

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中文摘要
翻译
听神经髓质细胞的细胞学及细胞间的联系 原子核将通过各种技术进行研究。 以下单个单元 记录侧支内或周围细胞的生理反应 猫的上级橄榄,辣根过氧化物酶(HRP)将 细胞外离子电渗。 逆行标记的细胞 所应用的HRP的运输将通过其位置来识别, 尼氏染色模式。 如有必要,将通过移除 HRP反应产物。 如果它不是已知的, 如此鉴定的细胞类别将使用高尔基体进行研究, 还原银染色、组织化学和免疫组化技术。 相同的 将对外侧上级内的细胞进行细胞学研究 橄榄。 研究的细胞学和细胞类之间的联系, 猫将提供与人类材料比较的基础, 将进行类似的细胞学和免疫组织化学研究。 通过 建立细胞类别的连接并表征它们 在猫的细胞学上,同样是细胞类别的连接 可以推断出人类的细胞学特征。 了解 这两种动物的听觉脑干的组织结构是这样的, 先进
英文摘要
The cytology and connection of cell classes in the medullary auditory nuclei will be studied by a variety of techniques. Following single unit recordings of physiological responses of cells in or around the lateral superior olive of the cat, horseradish peroxidase (HRP) will be iontophoresed extracellularly. The cells that are labelled by retrograde transport of the applied HRP will be identified by their locations and Nissl staining pattern. If necessary this will be accomplished by removal of the HRP reaction product. If it is not already known, the morphology of the cell classes that are so identified will be studied using Golgi, reduced silver, histochemical and immunohistochemical techniques. The same cytological studies will be done on cells within the lateral superior olive. Studies of the cytology and connections of the cell classes in the cat will provide the foundation for comparisons with human material in which similar cytological and immunohistochemical studies will be made. By establishing the connections of cell classes and characterizing them cytologically in the cat, the connections of cell classes that are likewise cytologically characterized in the human can be inferred. Understanding of the organization of the auditory brainstem in both species is therby advanced.
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