HISTOLOGY/SURGERY CORE

组织学/外科核心

• 批准号: 8071122
• 负责人: JOE C ADAMS
• 金额: $ 12万
• 依托单位: MASSACHUSETTS EYE AND EAR INFIRMARY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8071122
• 关键词: Acute; Analgesics; Anesthetics; Animal Model; Animals; Area; Auditory; Calibration; Cardiac; Cavia; Chinchilla (genus); Chronic; Clip; Complex; Data; Electron Microscopy; Electronics; Electrons; Equipment; Evaluation; Excision; Felis catus; Formvar 1285; Genotype; Hazardous Waste; Histological Techniques; Histology; Hospital safety; Human Resources; Individual; Laboratories; Learning; Light; Maintenance; Methods; Microscope; Microscopic; Monitor; Mus; Operative Surgical Procedures; Participant; Partner in relationship; Performance; Peripheral; Pharmaceutical Preparations; Physical environment; Physiological; Preparation; Procedures; Process; Protocols documentation; Rattus; Records Controls; Regulation; Research; Research Personnel; Safety; Schedule; Services; Slide; Solutions; Staining method; Stains; Structure; Students; Tail; Technical Expertise; Techniques; Thermometers; Time; Tissues; Training; Training Support; Weaning; Work; animal care; animal facility; base; cohort; experience; investigator training; mutant; neurophysiology; repaired; repository; research study; skills; surgical service; tissue preparation; tissue processing; tool

The Histology/Surgery Core provides the intellectual and physical environment in which Center Investigators, their trainees and support staff can learn and perform a variety of surgical and histological procedures. The Core will 1) Aims 1A, 2A: maintain existing shared facilities for animal surgery and histological processing, 2) Aims 1B, 2B: provide technical expertise and training in surgical techniques (both acute and chronic) and histological techniques for tissue processing of both peripheral and central auditory structures at the light- and electron-microscopic levels, 3) Aim 2D: continue to research improvements and refinements to histological and surgical methods, and 4) Aim 1E: assist with mouse husbandry in maintaining and propagating mutant lines. Some complex surgical techniques, such as preparation of the anesthetized cat for neurophysiological study, will be routinely offered as a technical service (Aims 1C, 1D). Some histological processing will be performed as a technical service, but only when task complexity and limited project scope make it inefficient to train the relevant group (Aim 2C). Facility maintenance will include 1) ordering of supplies, 2) preparation of stock solutions, 3) equipment repair, and 4) compliance with local and federal regulations. All research groups in the Center will benefit by the time saved in not duplicating these basic services. All investigators will also benefit from the repository of experience and expert advice the Core represents. First, significant time will be saved by offloading to Core personnel the task of training new students and new investigator-specific support staff. Second, Core expertise will enhance the research of all participants by 1) facilitating the incorporation of new techniques and 2) allowing them to use a wide range of techniques, each on a phasic basis, without having to maintain all the requisite skills within their own group. Core is staffed by one experienced individual with demonstrated expertise in all relevant areas, including animal surgery, immunohistpchemistry and a wide range of embedding and staining techniques for light- and electron-microscopic evaluation of peripheral and central auditory structures.

组织学/外科核心提供了智力和物理环境，中心 研究者、他们的受训人员和支持人员可以学习和执行各种手术和组织学 程序.核心将：1）目标1A、2A：维持现有的动物手术共用设施， 组织学处理，2）目标1B，2B：提供外科技术方面的技术专长和培训（两者都有 急性和慢性）和组织学技术，用于外周和中枢听觉 3）目标2D：继续研究改进， 组织学和手术方法的改进，以及4）目的1 E：协助小鼠饲养， 和繁殖突变株系。 一些复杂的手术技术，如准备麻醉猫的神经生理 研究，将作为技术服务定期提供（目标1C，1D）。一些组织学处理将 作为技术服务执行，但仅在任务复杂性和有限的项目范围使其效率低下时执行 培训相关群体（目标2C）。设施维护将包括1）订购供应品，2）准备 储备溶液，3）设备维修，以及4）遵守当地和联邦法规。所有研究 中心内的团体将受益于不重复这些基本服务而节省的时间。 所有调查人员还将受益于核心所代表的经验库和专家意见。 首先，通过将培训新学生和新员工的任务转移给核心人员， 专门的支持人员。第二，核心专业知识将通过以下方式加强所有参与者的研究：1） 促进新技术的结合，2）允许他们使用广泛的技术， 在阶段性的基础上，而不必在自己的群体中保持所有必要的技能。 核心工作人员由一名经验丰富的个人，在所有相关领域，包括 动物手术，免疫组化和广泛的包埋和染色技术， 外周和中枢听觉结构的电子显微镜评价。