AIDS VIRUS INFECTION OF HUMAN NEURONAL CELLS

艾滋病病毒感染与多种疾病有关 神经系统综合征，范围从周围神经病到 失智。 尽管人类免疫缺陷病毒（HIV）已 从脑组织和脑脊液中分离出来 感染患者，神经细胞病毒广泛传播的证据 复制尚未得到证实。 我们有初步证据表明限制了人类感染 神经母细胞瘤细胞发生在组织培养中。 我们将测试 假设限制性（或不完全）艾滋病毒感染发生在 神经元来源的细胞开发组织培养模型系统 用于研究艾滋病毒对人类脑细胞的影响。 这些研究将测试病毒基因表达的程度 一系列表型不同的神经母细胞瘤细胞类型。 我们 将评估细胞是否功能障碍，以 特定宿主细胞基因表达的改变，是通过以下方式改变的 感染艾滋病毒。 最后，我们将确定艾滋病毒感染是否会影响 每种神经母细胞瘤的分化能力，以及是否 细胞分化的水平改变了 HIV感染的纵容性。

AIDS virus infection has been associated with a variety of neurological syndromes ranging from peripheral neuropathy to dementia. Although the Human Immunodeficiency Virus (HIV) has been isolated from brain tissue and cerebro-spinal fluid of infected patients, evidence for wide spread nerve cell viral replication has not been demonstrated. We have preliminary evidence that restricted infection of human neuroblastoma cells occurs in tissue culture. We will test the hypothesis that restrictive (or incomplete) HIV infection occurs in cells of neuronal origin to develop a tissue culture model system for the study of HIV effects on human brain cells. These studies will test the extent of viral gene expression in a series of phenotypically different neuroblastoma cell types. We will assess whether cell dysfunction, measured in terms of alteration of specific host cell gene expression, is altered by infection with HIV. Finally, we will determine whether HIV infection affects the ability of each neuroblastoma to differentiate, and whether the level of cellular differentiation alters the degree of permissiveness of HIV infection.