AIDS VIRUS INFECTION OF HUMAN NEURONAL CELLS

艾滋病病毒感染人类神经细胞

• 批准号: 3411379
• 负责人: RUY SOEIRO
• 金额: $ 22.25万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3411379
• 关键词: AIDS; Retroviridae disease; brain cell; cell differentiation; cell type; defective virus; electron microscopy; flow cytometry; gene expression; genetic strain; genetic transcription; genetic translation; helper T lymphocyte; human immunodeficiency virus 1; human tissue; latent virus infection; messenger RNA; molecular pathology; neoplastic cell culture for noncancer research; nervous system disorder; neuroblastoma; neurologic manifestations; neurotropic virus; surface antigens; ultracentrifugation; virus DNA; virus RNA; virus cytopathogenic effect; virus genetics; virus infection mechanism; virus replication

AIDS virus infection has been associated with a variety of neurological syndromes ranging from peripheral neuropathy to dementia. Although the Human Immunodeficiency Virus (HIV) has been isolated from brain tissue and cerebro-spinal fluid of infected patients, evidence for wide spread nerve cell viral replication has not been demonstrated. We have preliminary evidence that restricted infection of human neuroblastoma cells occurs in tissue culture. We will test the hypothesis that restrictive (or incomplete) HIV infection occurs in cells of neuronal origin to develop a tissue culture model system for the study of HIV effects on human brain cells. These studies will test the extent of viral gene expression in a series of phenotypically different neuroblastoma cell types. We will assess whether cell dysfunction, measured in terms of alteration of specific host cell gene expression, is altered by infection with HIV. Finally, we will determine whether HIV infection affects the ability of each neuroblastoma to differentiate, and whether the level of cellular differentiation alters the degree of permissiveness of HIV infection.

艾滋病病毒感染与多种 神经系统综合征，从周围神经病变到 痴呆 虽然人类免疫缺陷病毒（HIV） 从脑组织和脊髓液中分离出来， 受感染的病人，广泛传播的神经细胞病毒的证据 复制尚未得到证实。 我们有初步证据表明，人类的限制性感染 神经母细胞瘤细胞发生在组织培养中。 我们将测试 假设限制性（或不完全）HIV感染发生在 神经元来源的细胞来开发组织培养模型系统 研究艾滋病病毒对人脑细胞的影响。 这些研究将测试病毒基因表达的程度， 一系列表型不同的神经母细胞瘤细胞类型。 我们 将评估细胞功能障碍， 特异性宿主细胞基因表达的改变， 感染艾滋病毒。 最后，我们将确定艾滋病毒感染是否会影响 每个神经母细胞瘤的分化能力，以及 细胞分化水平改变了 艾滋病毒感染的宽容。