AIDS VIRUS INFECTION OF HUMAN NEURONAL CELLS

艾滋病病毒感染人类神经细胞

• 批准号: 3411381
• 负责人: RUY SOEIRO
• 金额: $ 20.91万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3411381
• 关键词: AIDS; Retroviridae disease; antireceptor antibody; brain cell; cell differentiation; cell type; defective virus; electron microscopy; flow cytometry; gene expression; genetic strain; genetic transcription; genetic translation; helper T lymphocyte; human immunodeficiency virus 1; human tissue; latent virus infection; messenger RNA; molecular pathology; neoplastic cell culture for noncancer research; nervous system disorder; neuroblastoma; neurologic manifestations; neurotropic virus; transfection; ultracentrifugation; virus DNA; virus RNA; virus cytopathogenic effect; virus genetics; virus infection mechanism; virus protein; virus receptors; virus replication

AIDS virus infection has been associated with a variety of neurological syndromes ranging from peripheral neuropathy to dementia. Although the Human Immunodeficiency Virus (HIV) has been isolated from brain tissue and cerebro-spinal fluid of infected patients, evidence for wide spread nerve cell viral replication has not been demonstrated. We have preliminary evidence that restricted infection of human neuroblastoma cells occurs in tissue culture. We will test the hypothesis that restrictive (or incomplete) HIV infection occurs in cells of neuronal origin to develop a tissue culture model system for the study of HIV effects on human brain cells. These studies will test the extent of viral gene expression in a series of phenotypically different neuroblastoma cell types. We will assess whether cell dysfunction, measured in terms of alteration of specific host cell gene expression, is altered by infection with HIV. Finally, we will determine whether HIV infection affects the ability of each neuroblastoma to differentiate, and whether the level of cellular differentiation alters the degree of permissiveness of HIV infection.

艾滋病病毒感染已与多种疾病有关