EFFECTS OF PARVOVIRUS ON TEMPOROMANDIBULAR JOINT

这个项目的长期目标是培育一种动物 研究病毒引起的退行性改变的模型系统 颞颌关节。这项先导性研究旨在了解 金黄地鼠颞下颌关节可诱发病毒损伤 携带老鼠病毒的组织，这种病毒可产生颅面部和 仓鼠的牙齿异常。具体来说，仓鼠将是 接种了老鼠病毒，将在14、21或28岁时被处死 接种后天数。颞颌关节组织将 取出，然后进行光镜研究。 有代表性的组织切片将用苏木氧素染色 和曙红、马森三色、高碘酸席夫试剂或 为了研究间苯二酚品红的形态效应 颞颌关节组织中的病毒感染。如果 关节组织对病毒感染很敏感，那么我们计划 建议进行更多研究以确定涉及的特定细胞 在感染过程中使用DNA探针。此外，未来的研究将 使用放射自显影和形态测量技术来研究 病毒对胶原蛋白和弹性蛋白产生的影响以及 分发。这种方法可能会让我们更好地理解 特定的细胞参与了我们假设的关节病变 由病毒感染所致。

The long term objective of this project is to develop an animal model system to study viral induced degenerative changes in temporomandibular joints. This pilot study is designed to see if viral lesions can be induced in hamster temporomandibular joint tissues with a rat virus that is known to produce craniofacial and dental abnormalities in hamsters. Specifically, hamsters will be inoculated with rat virus and will be sacrificed at 14, 21, or 28 days post-inoculation. The temporomandibular joint tissues will be removed and then processed for light microscopic study. Representative tissue sections will be stained with hematoxyalin and eosin, Masson's trichrome, periodic acid Schiff's reagent or resorcin fuchsin in order to study the morphologic effects of the viral infection on the temporomandibular joint tissues. If the joint tissues are susceptible to the viral infection, then we plan to propose additional studies to determine the specific cells involved in the infection using DNA probes. In addition, future studies will employ autoradiographic and morphometric techniques to study the effects of the virus on collagen and elastin production and distribution. This approach may allow us to better understand the specific cells involved in the arthropathy that we hypothesize will result from viral infection.