CNS CCK IN APPETITIVE AND CONSUMMATORY FOOD MOTIVATION

CNS CCK 在食欲和完成性食物动机中的作用

• 批准号: 3410198
• 负责人: ELIOT STELLAR
• 金额: $ 6.29万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-25 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3410198
• 关键词: amygdala; area postrema; central nervous system; cerebellar nuclei; cerebral ventricles; cholecystokinin; drug administration routes; hypothalamus; injection /infusion; laboratory rat; motivation; neuroendocrine system; nutrient intake activity; satiations; solitary tract nucleus; taste

The purpose of the proposed studies is to determine the behavioral and biological bases of the effects of cholecystokinin octapeptide (CCK-8) on the rat's responses to food. The behavioral basis will be investigated by using appetitive as well as consummatory measures of motivated behavior. The appetitive measure is the running speed to very small food rewards in a meter-long runway, where postingestional effects are negligible. The consummatory measure is the amount of food ingested in a one-hour intake test in the animal's home cage. The consummatory measure gets at the satiety effects of CCK and the appetitive measure at the effects of CCK on taste-responsiveness taste reward, and the incentive value of foods. The biological basis will be investigated by pursuing our previous findings that exogenous CCK has both peripheral (intraperitoneal or i.p.) and central (intracerebroventricular or ICV) effects on satiation and on food rewards (1,2). To explore these effects further, prefeeding will be combined with peripheral or central CCK administration, testing the hypothesis that the effective dose in each route of administration will be lowered by prefeeding and a second hypothesis that subthreshold i.p. and ICV CCK doses will be additive and thus effective when given together. To explore the neural substrate, the locus of ICV injection will be varied from the anterior ventricles (third and lateral) to the fourth ventricle. In addition, specific sites in the brain will be investigated by lesioning those sites and by direct injection of CCK or the CCK-blocker, proglumide, in order to determine whether central (and where appropriate, peripheral) effects of CCK may be enhanced or diminished. In the study of the satiating effect of CCK, food-deprived rats will be used and the sites to be explored will include the area postrema (AP) and the paraventricular nuclei (PVN). In the study of the effects of CCK on taste reward and incentive effectiveness, food-satiated rats will be used to investigate sites in the limbic taste pathway, including the rostral region of the nucleus of the solitary tract (rostral NTS), the parabrachial nuclei (PBN) in the pontine taste area, the lateral hypothalamus (LH), and the central nucleus of the amygdala (CNA). By elucidating CCK's effects on both appetitive and consummatory aspects of the motivation toward food we will contribute to the theory of motivation, and also gain a better understanding of the behavioral and biological processes that go awry in the eating disorders.

拟议研究的目的是确定行为 缩胆囊素八肽作用的生物学基础 (CCK-8) 影响大鼠对食物的反应。 行为基础将 通过使用欲望和完成性进行调查 动机行为的测量。 食欲的衡量标准是 在一米长的跑道上以极小的食物奖励运行速度， 其中食后效应可以忽略不计。 圆满的 衡量标准是一小时摄入测试中摄入的食物量 在动物的笼子里。 完善措施达到 CCK 的饱腹感和食欲测量 CCK 对味觉反应性味觉奖励的影响，以及 食物的激励价值。 生物学基础将通过我们之前的研究来研究 研究结果表明，外源性 CCK 具有外周（腹膜内） 或 i.p.）和中枢（脑室内或 ICV）效应 饱腹感和食物奖励（1,2）。 探索这些影响 此外，预喂养将与外周或中枢相结合 CCK 管理，检验有效的假设 每种给药途径的剂量将通过喂食前降低 第二个假设是亚阈值 i.p。和 ICV CCK 剂量 当一起给予时，将是相加的，因此是有效的。 为了探索神经底物，ICV注射的位点将是 从前心室（第三和侧脑室）到 第四脑室。 此外，大脑中的特定部位将 通过损害这些部位并直接注射 CCK 或 CCK 阻滞剂丙谷胺，以确定 是否有中枢（以及适当情况下的外周）影响 CCK 可能增强或减弱。 在饱腹感的研究中 CCK 的影响，将使用禁食的大鼠，并且要进行的部位 探索的区域包括 postrema (AP) 和 室旁核（PVN）。 在CCK影响的研究中 对食物饱足大鼠的味觉奖励和激励效果的影响 将用于研究边缘味觉通路中的位点， 包括孤束核的喙区 （吻端NTS），脑桥味觉中的臂旁核（PBN） 下丘脑外侧区（LH）和中央核 杏仁核（CNA）。 通过阐明 CCK 对食欲和食欲的影响 我们将完成对食物的动机的各个方面 对动机理论做出贡献，也获得更好的 了解行为和生物过程 饮食失调出了问题。