AUTONOMIC NEURONS AND CARDIAC MUSCLE

建议研究钾通道基因表达的调控 在两种有助于心血管功能的细胞类型中，交感神经 节后神经元和心肌细胞。 在外周交感神经 在神经系统中，椎旁神经节中的神经元具有不同的放电 与椎前神经节中的特性相比，这是由于 大部分与电压激活钾的差异表达有关 渠道 具体目标1和3是针对比较 钾通道基因在交感神经元中的表达 类神经节，以找出哪些基因差异表达， 因此，可以有助于区分 电生理表型 具体目标2和3将比较 心房肌和心室肌钾通道表达。 公 确定动作电位的形状和持续时间明显 在心房和心室肌细胞中是不同的， 分化似乎是由于不同的表达， 电压门控钾通道 我们将确定哪些钾 通道基因在心房和心室肌中表达差异 肌肉. 这些研究将提供一个分子框架， 钾离子通道是如何影响 成人组织的分化，可以帮助制定战略， 寻找具有潜在治疗价值的新型钾通道拮抗剂 作为III类抗真菌药。

It is proposed to study the regulation of potassium channel gene expression in two cell types that contribute to cardiovascular function, sympathetic postganglionic neurons and cardiac myocytes. In the peripheral sympathetic nervous system, neurons in paravertebral ganglia have different firing properties compared with those in prevertebral ganglia and this is due in large part to differential expression of voltage-activated potassium channels. Specific Aims 1 and 3 are directed toward comparing the expression of potassium channel genes in sympathetic neurons in these two classes of ganglia to find which genes are differentially expressed and could therefore contribute to the differentiation of the electrophysiological phenotype. Specific Aims 2 and 3 will compare potassium channel expression in atrial and ventricular muscle. It is well established that the shape and duration of action potentials are markedly different in atrial and ventricular muscle cells, and much of this differentiation appears to be due to differential expression of voltage-gated potassium channels. We will determine which potassium channel genes are differentially expressed in atrial and ventricular muscle. These studies will provide a molecular framework for understanding how potassium channels contribute to the electrophysiological differentiation of adult tissues and could help develop strategies for finding new potassium channel antagonists with potential therapeutic value as class III anti-arrhythmics.