MENSTRUAL FUNCTION AND PHYSICAL AND MENTAL JOB STRESS

月经周期是女性生育潜力的标志，可以服务 监测从未尝试或实现怀孕的女工，但 其生殖功能和激素相关慢性疾病的风险可能 但还是会受到工作环境的影响。 此应用程序要求 用于调查女工月经功能和研究的资金 身心工作压力以及压力相关行为的影响 例如咖啡因摄入量、周期特征和卵巢激素 模式。 压力是一种普遍存在的职业暴露，并且 是可以预防或减少的。 已经被牵连到 月经失调，也被认为是一种可能的替代方案 暴露于其他环境的工人不良妊娠结局的解释 代理（例如 VDT）。 工作压力可能是女性面临的一个特殊问题， 他们对所谓的“第二班”的额外责任并没有 轻松地在非工作时间从压力中恢复过来。 该项目 因此，我们的建议解决了两个重要问题：（1）工作压力 女性 - 其影响需要严格调查的暴露 自己的优点，并帮助区分压力影响和其他影响 工作场所代理； (2) 开发生物标志物的必要性 月经功能，以识别和预防与工作相关的疾病 在非怀孕女性中（就像精液分析用于监测男性一样） 职业暴露）。 我们拟招收100名女护士，高低各50名 纪念斯隆-凯特琳癌症中心 (MSKCC) 的压力区域，可容纳三人 连续的月经周期。 受试者将被要求记录基础身体 温度，每天收集一小部分尿液并保持 结构化的月经日记。 为了评估排卵和黄体功能， 将分析尿液样本中的雌酮-3-葡萄糖醛酸苷 (E1G)， 孕二醇-3-葡萄糖苷酸 (pdG)、黄体生成素 (LH) 和肌酐 （铬）。 护士因工作岗位高而被选为目标人群 压力水平，以及高咖啡因摄入量和遵守意愿 似乎也有可能采用严格的养生法；进行了可行性研究 MSKCC 支持这一点。 受试者将由护士现场招募 MSKCC 现场总监将识别高压力和低压力单位 档案材料和与管理员讨论的基础。 数据分析将评估与工作相关的强度之间的关系 压力源、咖啡因摄入和抗肿瘤药物暴露，以及 月经功能的几种测量（周期长度、排卵和黄体 相位充足性）。 E1G 和 PdG 的绝对水平和变化率将 还可以根据暴露程度进行比较。 最后，为了帮助设计 未来的月经研究、基础体温的有效性和 将评估前瞻性确定的月经周期报告， 使用泌尿激素数据作为金标准。

The menstrual cycle is a marker of female fertility potential and can serve to monitor female workers who never attempt or achieve a pregnancy, but whose reproductive function and risk of hormone-related chronic disease may nonetheless be affected by the work environment. this application requests monies to investigate menstrual function in women workers and to study effects of physical and mental job stress, and stress-related behaviors like caffeine consumption, on cycle characteristics and ovarian hormone patterns. Stress i s pervasive occupational exposure and one that potentially can be prevented or reduced. It has been implicated in menstrual disorders and has also been cited as a possible alternative explanation for adverse pregnancy outcomes among workers exposed to other agents (e.g. VDTs). Job stress may be a particular problem for women, whose added responsibility for the so-called "second shift" does not readily allow for recovery from stress during nonwork hours. the project we are proposing thus addresses two important issues: (1) job stress in females - an exposure whose effect needs rigorous investigation both on its own merits and to help disentangle stress effects from effects of other work place agents; and (2) the need to develop biological markers of menstrual function in order to identify and prevent work-related disorders among non-pregnant women (much as semen analysis has served to monitor male occupational exposures). We propose to enroll 100 female nurses, 50 each drawn from high and low stress areas of Memorial Sloan-Kettering Cancer Center (MSKCC), for three consecutive menstrual cycles. Subjects will be asked to record basal body temperature, collect a small aliquot of urine daily and maintain a structured menstrual diary. To evaluate ovulatory and luteal function, urine samples will be analyzed for estrone-3-glucuronide (E1G), pregnanediol-3-glucuronide (pdG), luteinizing hormone (LH) and creatinine (Cr). Nurses were selected as the target population because of high job stress levels, and because high caffeine intake and willingness to comply with a demanding regimen also seemed likely; a feasibility study conducted at MSKCC supports this. Subjects will be recruited by a nurse-field director on-site at MSKCC who will identify high and low stress units on the basis of archival material and discussions with administrators. Data analyses will evaluate the relations in the intensity of job-related stressors, caffeine consumption, and exposure to antineoplastics, and several measures of menstrual function (cycle length; ovulation; and luteal phase adequacy). Absolute levels and rates of change in E1G and PdG will also be compared in relation to exposure. Finally, to aid in the design of future menstrual studies, the validity of basal body temperature and prospectively ascertained reports of menstrual cycles will be assessed, using the urinary hormone data as a gold standard.