FIBRONECTIN RECEPTOR MEDIATED ADHESION OF P GINGIVALIS

大约75%的成年人口患有某种形式的 牙周病。当有毒的革兰氏菌引起牙周病 负性细菌寄生在牙龈缝隙中。这一殖民统治 涉及细菌粘连和上皮细胞之间的特定相互作用 细胞表面受体。在血清、唾液和 沟液也可以参与这些粘连事件。 牙龈卟啉单胞菌是一种革兰氏阴性厌氧菌，即 通常从牙周袋中分离出来。其口服液的坚持 上皮细胞已有很好的文献记载，但这种相互作用的分子基础 是未知的。已知的纤维连接蛋白参与了许多重要的 涉及整合素受体家族的黏附事件，以及一些 这些受体已经在上皮细胞上被识别出来。此外, 纤维连接蛋白可在血管表面以不溶形式表达。 上皮细胞，并由它们分泌。此外，可溶性纤维连接蛋白是 在唾液和血清中都有发现。最后，几种细菌菌株已经 已被鉴定出能够结合各种形式的人纤维连接蛋白。 因此，这项建议的目的是确定纤维连接蛋白的作用 在牙龈假单胞菌与口腔上皮细胞的黏附中。一旦这个角色 纤维粘连蛋白在细菌粘连中的确定，预防性治疗 可以被开发出来，以抑制这种最初的殖民事件。 因此，这项提案的具体目标是：(1) 牙龈假单胞菌与纤维连接蛋白的相互作用：(2)鉴定 口腔黏膜纤维连接蛋白表达和/或结合能力的研究 以及(3)确定纤维连接蛋白在血管内皮细胞中的潜在作用。 牙龈假单胞菌与口腔上皮细胞的结合。 牙周炎假单胞菌与可溶性和/或 不溶性纤维结合蛋白将通过酶联免疫吸附试验进行测定。两者都是 上皮细胞株和新鲜分离的口腔上皮细胞 用流式细胞仪分析确定细胞表面的存在 纤维连接蛋白，纤维连接蛋白受体，以及这些受体的能力 结合纤维连接蛋白。将进行粘附性分析以确定 牙龈假单胞菌与口腔上皮细胞的结合特性。重要的是， 将已建立的细胞系用作细菌的体外模型的能力 口腔内的粘连也将被调查。

Approximately 75% of the adult population suffers from some form of periodontal disease. Periodontal disease results when virulent gram negative bacteria colonize the gingival crevice. This colonization involves specific interactions between bacterial adhesions and epithelial cell surface receptors. Secreted molecules found in serum, saliva, and crevicular fluid can also participate in these adhesion events. Porphyromonas gingivalis is a gram negative obligate anaerobe that is commonly isolated from periodontal pockets. Its adherence to oral epithelium is well documented, but the molecular basis for this interaction is unknown. Fibronectin is known to participate in a number of important adhesion events involving the integrin family of receptors, and a number of these receptors have been identified on epithelial cells. In addition, fibronectin can be expressed in an insoluble form on the surface of epithelial cells and also secreted by them. Also, soluble fibronectin is found in both saliva and serum. Finally, several bacterial strains have been identified which are able to bind various forms of human fibronectin. Thus the objective of this proposal is to determine the role of fibronectin in the adhesion of P. gingivalis to oral epithelial cells. Once the role of fibronectin in bacterial adhesion is determined, preventive therapies could to be developed which would inhibit this initial colonization event. Accordingly, the specific aims of this proposal are: (1) to characterize the interactions between P. gingivalis and fibronectin; (2) to characterize the fibronectin expression and/or fibronectin binding capacity of oral epithelium; and (3) to determine the potential role of fibronectin in the binding of P. gingivalis to oral epithelium. The ability of P. gingivalis to interact with either soluble and/or insoluble fibronectin will be determined by means of an ELISA. Both an epithelial cell line and freshly isolated oral epithelial cells will be analyzed by flow cytometry to determine the presence of cell surface fibronectin, receptors for fibronectin, and the ability of the receptors to bind fibronectin. Adhesion assays will be performed to determine the binding properties of P. gingivalis and oral epithelium. Importantly, the ability to use an established cell line as an in vitro model for bacterial adhesion in the oral cavity will also be investigated.