DYNAMIC STRUCTURE OF MEMBRANE GALACTOLIPIDS

膜半乳糖脂的动态结构

• 批准号: 3438439
• 负责人: PHOEBE K DEA
• 金额: $ 6.84万
• 依托单位: CALIFORNIA STATE UNIVERSITY LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3438439
• 关键词: calorimetry; chlorophyll; chloroplasts; conformation; galactolipids; lipid structure; membrane lipids; nuclear magnetic resonance spectroscopy; photosynthesis

Photosynthesis, the conversion of light energy to useful chemical energy, is a membrane process. The light-harvesting events of photosynthesis are known to take place in the thylakoid membrane. While the function of chlorophylls in the photosynthetic process is reasonably well defined, the structural organization of the light-harvesting chlorophylls in the thylakoid membrane is a subject of considerable current interest. Possible interactions between chlorophyll and galactolipids in the thylakoid membrane are of particular interest since galactolipids are the major lipid of this membrane. This study proposes the use of deuterium nuclear magnetic resonance (H-2 NMR) as the main spectroscopic technique to study the structure of the galactolipids, and to investigate specific interactions between chlorophyll a and the galactolipids. Selectively deuterated galactolipids will be synthesized by catalytic reduction of the unsaturated galactolipids isolated from the thylakoid membrane. Most H-2 NMR work has so far focused on phospholipid-containing membranes, and essentially no research has been reported on galactolipids, some of which are of considerable clinical relevance. In addition to NMR, other techniques, such as differential scanning calorimetry, are utilized to provide useful complementary information. The proposed research extends previous work on the organization of chlorophyll a in bilayer membranes. This study should yield useful information on the structure, interaction, phase behavior and dynamics of the galactolipids and chlorophyll a in model membrane systems.

光合作用，将光能转化为有用的化学能， 是一个膜过程。 光合作用的光捕获事件是 发生在类囊体膜上。 虽然功能 叶绿素在光合作用过程中是合理的定义， 光捕获叶绿素的结构组织， 类囊体膜是当前相当感兴趣的课题。 可能 类囊体中叶绿素与半乳糖脂的相互作用 由于半乳糖脂是主要的脂质， 这个膜。 这项研究提出了使用氘核磁共振（H-2 NMR）作为主要光谱技术来研究化合物的结构。 半乳糖脂，并研究叶绿素之间的特定相互作用 a和半乳糖脂。 选择性氘代半乳糖脂将是 通过催化还原不饱和半乳糖脂合成 分离自类囊体膜。 到目前为止，大多数H-2 NMR工作都集中在 在含磷脂的膜上，基本上没有研究 报道了半乳糖脂，其中一些具有相当大的临床意义 本案无关 除了NMR之外，其他技术，例如微分 扫描量热法，用于提供有用的补充 信息. 拟议的研究扩展了以前关于组织的工作， 双层膜中的叶绿素a。 这项研究将产生有益的结果。 关于结构、相互作用、相行为和动力学的信息 模型膜系统中的半乳糖脂和叶绿素a。

项目成果

Hydrophobic mismatch in gramicidin A'/lecithin systems.

短杆菌肽 A/卵磷脂系统中的疏水错配。

• DOI: 10.1021/bi00478a015
• 发表时间: 1990
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Watnick,PI;Chan,SI;Dea,P
• 通讯作者: Dea,P

Characterization of the transverse relaxation rates in lipid bilayers.

脂质双层横向弛豫率的表征。

• DOI: 10.1073/pnas.87.6.2082
• 发表时间: 1990
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Watnick,PI;Dea,P;Chan,SI
• 通讯作者: Chan,SI