NMR STUDIES OF CHLOROPHYLL A IN PHOTOSYNTHETIC MEMBRANE

叶绿素参与了将阳光转化为 光合作用过程中的生化能。 只有 一小部分叶绿素参与了 光化学反应 剩下的叶绿素 执行一个支持性的，但基本的功能，收集 反应中心所需的能量。 虽然功能 叶绿素在光合作用过程中是相当好的 定义，光捕获的结构组织 类囊体膜中的叶绿素是一个相当重要的课题。 当前兴趣 这项研究提出了使用高场核磁共振 共振光谱（NMR）研究结构和动力学 完整的类囊体膜中的脂质，和特定的 叶绿素和脂质之间的相互作用。 一个系统 研究了酰基链长，不饱和度， 以及模型磷脂双层中极性头基的变化 叶绿素a与双层膜的相互作用 进行切割以产生比较信息。 辅助 技术将包括光谱学和色谱法， 分离、纯化和纯度测定的必要条件 叶绿素和脂质，并将包括差示扫描 量热法作为NMR技术的辅助手段。 拟议的研究扩展了以前开展的工作， 组织叶绿素a的供资期 二肉豆蔻酰磷脂酰胆碱双层膜和 单半乳糖基二酰基甘油，一种主要的半乳糖脂， 类囊体膜 这项研究应该提供有用的信息， 类囊体膜的结构以及相互作用， 叶绿素a在模型膜中的相行为和动力学 系统.

Chlorophylls are involved in the conversion of sunlight into biochemical energy during the process of photosynthesis. Only a small fraction of the chlorophyll present participates in the basic photochemistry reaction. The remaining bulk of the chlorophylls performs a supportive but essential function of collecting the energy required by the reaction center. While the function of chlorophylls in the photosynthetic process is reasonably well defined, the structural organization of the light-harvesting chlorophylls in the thylakoid membrane is a subject of considerable current interest. This study proposes the use of high-field nuclear magnetic resonance spectroscopy (NMR) to study the structure and dynamics of lipids in the intact thylakoid membrane, and the specific interactions between chlorophyll and the lipids. A systematic study on the effects of acyl chain length, degree of unsaturation, and changes in polar headgroup in the model phospholipid bilayer on the interaction of chlorophyll a with the bilayer membrane will be carried cut to yield comparative information. Ancillary techniques will include optical spectroscopy and chromatography as requisite for the isolation, purification and purity determination of chlorophylls and lipids, and will include differential scanning calorimetry as adjunct to the NMR technique. The proposed research extends work carried out during the previous funding period on the organization of chlorophyll a in dimyristoylphosphatidylcholine bilayer membranes and in monogalactosyldiacylglycerols, a major galactolipid found in the thylakoid membrane. This study should yield useful information on the structure of the thylakoid membrane as well as the interaction, phase behavior and dynamics of chlorophyll a in model membrane systems.