SODIUM MRI & CSI OF EXPERIMENTAL DIABETIC RETINOPATHY

拟议的研究的具体目的是使用钠磁铁 磁共振成像(23Na MRI)和钠化学位移成像(23Na CSI) 在两种动物模型上观察玻璃体的状态 糖尿病视网膜病变。糖尿病视网膜病变是糖尿病的一种并发症 25岁至35岁人群新增失明的主要原因 几年来，随着医学的进步，它的重要性继续增长 糖尿病患者的长寿。越来越多的糖尿病盲人， 特别是在青少年发病类别中，对 制定有效的策略，以基本了解、诊断和 治疗这种疾病的方法。 磁共振已被证明是一种很好的研究工具。 钠，因为~(23)Na磁共振可获得高对比度 图像，这个四极核对其固有的敏感性 环境，以及生物体内相对较高的钠浓度 系统。在这项工作中，我们将使用钠磁共振成像和 首席研究员开发的化学位移成像技术 和她的学生和合作者一起研究糖尿病的影响 玻璃体视网膜病变的原因是： 1.在玻璃体内局部测量钠自旋 松弛时间和钠“能见度”； 2.评估血液成分在血液-视网膜中的渗漏 通过监测静脉内给药顺磁性药物的流动来实现屏障 将试剂移入玻璃体。 糖尿病视网膜病变将通过激光光凝建立模型 视网膜和四氧嘧啶诱导的糖尿病。我们预计会有血- 视网膜屏障功能障碍将导致化学位移图像 玻璃体的区域与正常玻璃体的区域不同。我们也 预计钠自旋-自旋弛豫时间和能见度 会随着玻璃体状态的改变而改变 视网膜血管中的血液成分。这些参数将 洞察相关玻璃体凝胶性质的变化 患有糖尿病视网膜病变。

The specific aim of the proposed research is to use sodium magnetic resonance imaging (23Na MRI) and sodium chemical shift imaging (23Na CSI) to characterize the state of the vitreous body in two animal models of diabetic retinopathy. Diabetic retinopathy, a complication of diabetes, is the major cause of new blindness in persons between the ages of 25 and 35 years, and continues to grow in importance as medical advances extend the longevity of diabetics. The increasing number of diabetic blind, especially in the juvenile onset category, offers a clear challenge to develop effective strategies for the basic understanding, diagnoses and treatment of this disorder. Magnetic resonance has proven to be an excellent tool for the study of sodium because of the high contrast obtainable in 23Na magnetic resonance images, the inherent sensitivity of this quadrupolar nucleus to its environment, and the relatively high concentration of sodium in biological systems. In this work we will use sodium magnetic resonance imaging and chemical shift imaging techniques developed by the principal investigator and her students and collaborators to investigate the effects of diabetic retinopathy on the vitreous body by: 1. measuring, on a local basis within the vitreous, sodium spin-spin relaxation times and sodium "visibility"; 2. assessing leakage of blood constituents across the blood-retinal barrier by monitoring the flow of intravenously administered paramagnetic shift reagent into the vitreous. Diabetic retinopathy will be modeled by laster photocoagulation of the retina and by alloxan induction of diabetes. We anticipate that blood- retinal barrier dysfunction will result in chemical shift images of the regions of the vitreous distinct from those of normal vitreous. We also anticipate that sodium spin-spin relaxation times and visibility values will change as the state of the vitreous is altered through the leakage of blood constituents from retinal blood vessels. These parameters will provide insight into the changing nature of the vitreous gel associated with diabetic retinopathy.