GENOME ANALYSIS OF THE GENUS BDELLOVIBRIO

人们对蛭弧菌的遗传学知之甚少，而且有一种系统可以 将DNA引入蛭弧菌是最近才有报道的。这个 绘制蛭弧菌染色体图谱将作为一种 为更多的基因研究提供刺激和框架 基因数据是可以协调的。总体目标是 构建细菌性蛭弧菌基因组物理图谱，以 比较了几株蛭弧菌的物理图谱，并确定了 地图上的一些基因。高质量、未剪裁的基因组DNA将 从细菌性蛭弧菌109J和其他几种细菌中提取 菌株，制成琼脂糖珠。DNA将被罕见的切割 限制性内切酶和由此产生的大DNA片段 采用脉冲场凝胶电泳法进行分离。限制 不同菌株的模式或指纹将按顺序进行比较 以确定亲缘关系和分类学意义。限制 碎片将相对于彼此进行排序。这将是 通过1)使用一种酶的片段来探查Southern 不同酶限制性内切酶图谱的杂交以发现重叠 片段间隔期；2)用DNA探针技术检测蜈蚣弧菌基因组YAC克隆 分离的基因组限制性片段。基因图谱的开端 将通过定位克隆的基因和转座子插入而产生 在物理地图上。

Little is known about the genetics of the bdellovibrios, and a system for introducing DNA into bdellovibrios has only recently been reported. The development of a map of the bdellovibrio chromosome would serve as a stimulus for additional genetic studies and a framework onto which genetic data could be coordinated. The overall objectives are to construct a physical map of the Bdellovibrio bacteriovorus genome, to compare the physical maps of several bdellovibrio strains, and to locate a number of genes on the map. High quality, unsheared genomic DNA will be extracted from Bdellovibrio bacteriovorus 109J, and several other strains, into agarose beads. The DNA will be cut with rare cutting restriction endonucleases, and the resulting large DNA fragments separated using pulsed field gel electrophoresis. The restriction patterns, or fingerprints, of various strains will be compared in order to determine relatedness and taxonomic significance. Restriction fragments will be ordered relative to each other. This will be accomplished by 1)using fragments of one enzyme digest to probe Southern blots of different enzyme restriction patterns to discover overlaps between fragments; 2)probing Bdellovibrio genomic YAC clones with isolated genomic restriction fragments. The beginnings of a genetic map will be originated by positioning cloned genes and transposon insertions on the physical map.