INSERTION OF PORINS INTO PREY MEMBRANES BY BDELLOVIBRIOS

蛭弧菌物种破坏其猎物细胞的机制 将检查细胞质膜。 一些原核生物和 已知欧洲寄生虫能够插入寄生虫- 将其衍生的蛋白质导入宿主的细胞质膜。 在一些 例如，这种易位蛋白可能在 寄生机制。 蛭弧菌与它们的 被捕食细胞可以用作研究这种相互作用的模型。 初步数据表明蛭弧菌能够 一种蛋白质进入叶绿体细胞质膜，这种蛋白质与 在带有OmpF的SDS-PAGE凝胶上迁移，OmpF是一种主要的E. coli外膜孔蛋白。 易位蛋白的来源和结构将被用作猎物 以确定是否存在易位所需的特定Omp。 使用 放射性标记，易位蛋白质的来源将被研究， 以及其插入到原生质体细胞质中的动力学 膜的 一旦蛋白质的来源和结构被确定， 确定，其定位显微镜和单克隆抗体标记 蛋白A-胶体金复合物。

The mechanism by which Bdellovibrio species breach their prey cell's cytoplasmic membrane will be examined. A number of both prokaryotic and eurkaryotic parasites are known to be capable of inserting a parsite- derived protein into the cytoplasmic membrane of their host. In some instance, this translocated protein may play a significant role in the mechanism of parasitism. The interaction of the bdellovibrios with their prey cell may be used as a model for studying such interactions. Preliminary data indicates that the bdellovibrios are able to translocate a protein into the bdelloplast cytoplasmic membrane, and this protein co- migrates on SDS-PAGE gels with OmpF, a major E. coli outer membrane porin. The source and structure of the translocated protein will be used as prey to determine if there is a specific Omp required for translocation. Using radioactive labels, the source of the translocated protein will be studied, as well as the kinetics of its insertion into the bdelloplast cytoplasmic membrane. Once the source and structure of the protein has been determined, its localization microscopy and monoclonal antibodies tagged wit protein A-colloidal gold complexes.