INSERTION OF PORINS INTO PREY MEMBRANES BY BDELLOVIBRIOS

蛭弧菌将孔蛋白插入猎物膜中

• 批准号: 3438832
• 负责人: JOHN J TUDOR
• 金额: $ 11.23万
• 依托单位: SAINT JOSEPH'S UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-29 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3438832
• 关键词: Escherichia coli; Vibrio; cytoplasm; host organism interaction; membrane proteins; monoclonal antibody; pore forming protein; protein structure function; protein transport

The mechanism by which Bdellovibrio species breach their prey cell's cytoplasmic membrane will be examined. A number of both prokaryotic and eurkaryotic parasites are known to be capable of inserting a parsite- derived protein into the cytoplasmic membrane of their host. In some instance, this translocated protein may play a significant role in the mechanism of parasitism. The interaction of the bdellovibrios with their prey cell may be used as a model for studying such interactions. Preliminary data indicates that the bdellovibrios are able to translocate a protein into the bdelloplast cytoplasmic membrane, and this protein co- migrates on SDS-PAGE gels with OmpF, a major E. coli outer membrane porin. The source and structure of the translocated protein will be used as prey to determine if there is a specific Omp required for translocation. Using radioactive labels, the source of the translocated protein will be studied, as well as the kinetics of its insertion into the bdelloplast cytoplasmic membrane. Once the source and structure of the protein has been determined, its localization microscopy and monoclonal antibodies tagged wit protein A-colloidal gold complexes.

蛭弧菌破坏猎物细胞的机制 将检查细胞质膜。 许多原核生物和 已知真核寄生虫能够插入寄生虫 衍生的蛋白质进入宿主的细胞质膜。 在一些 例如，这种易位蛋白可能在 寄生机制。 蛭弧菌与其相互作用 猎物细胞可以用作研究这种相互作用的模型。 初步数据表明，蛭弧菌能够易位 一种蛋白质进入蚓状体细胞质膜，并且这种蛋白质共同 与 OmpF（一种主要的大肠杆菌外膜孔蛋白）一起在 SDS-PAGE 凝胶上迁移。 易位蛋白的来源和结构将被用作猎物 以确定易位是否需要特定的 Omp。 使用 放射性标记，将研究易位蛋白质的来源， 以及其插入蚓状体细胞质的动力学 膜。 一旦确定了蛋白质的来源和结构 确定，其定位显微镜和单克隆抗体标记 机智蛋白 A-胶体金复合物。

项目成果

Translocation of an outer membrane protein into prey cytoplasmic membranes by bdellovibrios.

蛭弧菌将外膜蛋白易位到猎物细胞质膜中。

• DOI: 10.1128/jb.176.4.948-952.1994
• 发表时间: 1994
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Tudor,JJ;Karp,MA
• 通讯作者: Karp,MA