BIOSYNTHETIC STUDIES OF EUDISTOMINS C E K AND L

这项研究的目的是阐明生物合成的起源， 从奥沙西平(OTAP)安非他明的初级代谢物 在海鞘中，橄榄球藻属。这些有效的抗病毒(HSV， Vaccina)代谢物似乎来源于色胺、半胱氨酸和 S-腺苷蛋氨酸，尽管考虑了替代前体。 沿着生物合成路径的潜在中间体被识别并 提出了这些中间体的放射性标记合成方法。vbl.使用 以前开发的细胞-FREE方法学是为了研究其他植物的生物合成 殖民地海洋无脊椎动物，这些假定的前体将被测试 纳入OTAP优地塞米松。双标签和可降解性 将采用各种方案来确定前兆的特异性和 从而排除可能导致合并的分解代谢过程 放射性。OTAP的生物合成研究结果 优地沙星可能会导致后续的研究，目的是识别 乳杆菌中编码这些代谢物的遗传物质。 通过基因工程的开发可以使商业上可用 这些潜在药物的数量，消除了有限的问题 自然来源的可用性。

The objective of this research is to elucidate the biosynthetic origin, from primary metabolites, of the oxathiazepine-bearing (OTAP) eudistomins in the ascidian Eudistoma olivacium. These potently antiviral (HSV, Vaccina) metabolites seemingly derive from tryptamine, cysteinal and S-adenosylmethionine, although alternative precursors are considered. Potential intermediates along the biosynthetic path are identified and radiolabelled syntheses of these intermediates are proposed. Using cell-frree methodology previously developed to study biosynthesis in other colonial marine invertebrates, these putative precursors will be tested for incorporation into the OTAP eudistomins. Double-label and degradative schemes will be employed to determine the specificity of the precursors and thus rule out catabolic processes that might lead to incorporation of radioactivity. The results of this biosynthetic study of the OTAP eudistomins could lead to subsequent studies aimed at identification of genetic material in E. olivacium that codes for these metabolites. Exploitation via genetic engineering could then make available commercial quantities of these potential drugs, obviating the problem of limited natural-source availability.