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HORMONAL CONTROL OF INGESTIVE BEHAVIORS

摄入行为的荷尔蒙控制

基本信息

批准号：
3440853
负责人：
PETER C BUTERA
金额：
$ 4.65万
依托单位：
NIAGARA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-05-01 至 1990-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3440853
关键词：
brain mapping eating estradiol estrogens hormone regulation /control mechanism laboratory rat neuroanatomy neurons nutrition related tag paraventricular nucleus protein biosynthesis

项目摘要

Previous work has shown that the paraventricular nucleus of the hypothalamus (PVN) is a critical brain site for the control of feeding behavior. In addition, we and others have found that estrogenic stimulation of the PVN is sufficient to lower food intake and body weight in ovariectomized females. The proposed experiments will extend the investigation of the neuroanatomical locus of the estrogenic suppression of food intake, and will also examine a possible biochemical mechanism underlying this hormone-behavior relationship. Specifically, it is hypothesized that the effects of estorgens on eating can be attenuated by lesions of the PVN, and that the estrogenci suppression of food intake is dependent upon changes in protein synthesis within neurons of the PVN. It is also hypothesized that lesions of the PVN will not prevent the facilitation of lordosis by estradiol. Experiment 1 will evaluate the behavioral responsiveness of ovariectomized rats with PVN lesions to peripheral injections of estradiol. In experiment 2, inhibitors of protein or RNA synthesis (e.g., anisomycin, actinomycin-D) will be directly applied to the PVN of ovariectomized rats given peripheral injections of estradiol. In this way we will be able to evaluae the importance of protein synthesis within the PVN for estrogen-iduced changes in eating, and investigate one possible biochemical mechanism underlying this phenomenon. The results of these experiments will hopefully confirm and extend previous findings which indicate that the PVN is an important brain region for the estrogenic suppression of food intake. Future studies could then examine the interactions between estrogens, catecholamines, and neuropeptides in the PVN so that the exact biochemical substrate for the effects of estrogens on feeding could be more clearly specified. These experiments have important implications for understanding the mechanisms underlying endocrine-related eating disorders such as anorexia nervosa and some forms of obesity. In addition, information from these studies will contribute to our understanding of brain mechanisms involved in feeding behavior.

先前的工作表明，脑室旁核下丘脑(PVN)是控制脑部运动的关键部位。进食行为。此外，我们和其他人发现，下丘脑室旁核的雌激素刺激足以降低食物去卵巢女性的摄入量和体重。建议数实验将扩大对神经解剖学的研究雌激素抑制食物摄入量的部位，也会研究一种可能的生物化学机制荷尔蒙与行为的关系。具体地说，它是假设的雌激素对进食的影响可以通过以下方式减弱病变的PVN，并认为雌激素抑制食物摄入量取决于体内蛋白质合成的变化室旁核神经元。它还假设，皮肤的损伤 PVN不能阻止雌二醇促进前凸的发生。实验1将评估被试的行为反应去卵巢大鼠PVN病变外周注射雌二醇。在实验2中，蛋白质或RNA合成的抑制剂 (例如，茴香霉素、放线菌素-D)将直接应用于卵巢切除大鼠外周注射马桑内酯后的下丘脑室旁核雌二醇。通过这种方式，我们将能够评估下丘脑室旁核内的蛋白质合成与雌激素引起的变化并研究一种可能的生化机制这一现象背后的原因。这些实验的结果将有望证实和扩展之前的发现，这些发现表明下丘脑室旁核是雌激素的重要脑区抑制食物摄取。未来的研究可能会检查雌激素、儿茶酚胺和 PVN中的神经肽使准确的生化底物因为雌激素对进食的影响可能更清楚指定的。这些实验具有重要的意义了解内分泌相关的机制进食障碍，如神经性厌食症和某些形式的肥胖。此外，来自这些研究的信息将有助于我们理解脑机制涉及的进食行为。