PROSTAGLANDINS AND VASOPRESSIN ACTION

目前的研究方案旨在定义生化机制。 参与抗利尿激素(血管加压素)和 肾脏中的前列腺素。绕过组织的问题 在大多数以前的研究中遇到的异质性，这种相互作用将 在集合管中进行特殊检查，显微解剖自 大鼠肾脏的内髓。这项提案的具体目标将是 是：1)a)确定内源抑制的效果 前列腺素合成对血管加压素(AVP)依赖性cAMP形成的影响 收集小管，b)以确定高渗透压的影响和 特定溶质对集合管前列腺素合成的影响 它们对AVP依赖的cAMP形成的影响，c)评估 钙在前列腺素和前列腺素相互作用中的潜在作用 加压素。2)确定AVP是否刺激前列腺素 在集合管中合成，如果是，确定其机制 刺激的力量。3)确定前列腺素合成是否发生改变 集合管可能与受损的特定证候有关 水的排泄。

The present research proposal aims to define the biochemical mechanisms involved in the interaction between antidiuretic hormone (vasopressin) and prostaglandins in the kidney. To circumvent the problem of tissue heterogeneity encountered in most previous studies, this interaction will be examined specifically in the collecting tubule microdissected from the inner medulla of the rat kidney. The specific aims of this proposal will be: 1) a) To determine the effects of inhibition of endogenous prostaglandin synthesis on vasopressin (AVP)-dependent cAMP formation in the collecting tubule, b) to determine the effects of hyperosmolality and specific solutes on prostaglandin synthesis in the collecting tubule and their implications for AVP-dependent cAMP formation, c) to assess the potential role of calcium in the interaction between prostaglandins and vasopressin. 2) To determine whether AVP stimulates prostaglandin synthesis in the collecting tubule, and if so, to determine the mechanism of stimulation. 3) To determine whether altered prostaglandin synthesis in the collecting tubule may be associated wih specific syndromes of impaired water excretion.