SCHIOTOSOME-SNAIL COMPATIBILITY: UNDERLYING MECHANISMS

这项研究旨在提供对所使用的机制的理解 曼氏血吸虫在中间宿主中生存和繁殖 钉螺Biomphalaria grarata。曼氏杆菌是一种人类致病因子。 血吸虫病是一种严重危害人类健康的疾病 热带的许多地区。在此前提下，S. 曼氏杆菌感染光滑假单胞菌是一种高度特异的现象，而且 由于在界面上发生的事件而表现出专一性 对于带有宿主-蜗牛血细胞质膜的孢子囊，我们的目标是 提供关于特异性的细胞化学基础的信息。 实验将被设计成产生支持或 驳斥与以下问题直接相关的各种假设： (A)蜗牛血细胞识别吸虫孢子囊的机制 (二)蜗牛增强抵抗力的机制， 或较不特异的性质，以吸虫孢子囊 (C)吸虫孢子囊避免被 某些钉螺品系的血细胞 (D)吸虫孢子囊干扰先天的机制 钉螺的抗药性 方法学将涉及血吸虫孢子囊幼虫的体外培养 与宿主钉螺具有免疫活性的白细胞(血细胞)一起。两者都有 敏感和耐药的蜗牛品系将被用作 血细胞。宿主潜在重要分子的敏感标记 和寄生虫的来源将使我们能够评估它们在调解 兼容性。

This research is aimed at providing an understanding of the mechanisms used by Schistosoma mansoni to survive and multiply in its intermediate host snail Biomphalaria glabrata. S. mansoni is a causative agent of human schistosomiasis, a serious debilitating disease in human populations in many areas of the tropics. On the premise that the compatibility of S. mansoni with B. glabrata is a highly specific phenomenon, and that the specificity is manifested as a result of events occurring at the interface of the sporocyst with host-snail hemocyte plasma membranes, we aim to provide information on the cellular-chemical basis of specificity. Experiments will be designed to yield information which will support or refute various hypotheses that bear directly on the following issues: (a) The mechanisms by which snail hemocytes recognize trematode sporocysts (b) The mechanisms by which snails acquire enhanced resistance, of a more or less specific nature, to trematode sporocysts (c) The mechanisms by which trematode sporocysts avoid destruction by hemocytes of certain snail strains (d) The mechanisms by which trematode sporocysts interfere with innate resistance of snails Methodologies will involve in vitro culture of schistosome sporocyst larvae together with immunocompetent leukocytes (hemocytes) of host snails. Both susceptible and resistant strains of snail will be used as sources of hemocytes. Sensitive labeling of potentially important molecules of host and parasite origin will permit us to evaluate their roles in mediation of compatibility.