SCHIOTOSOME-SNAIL COMPATIBILITY: UNDERLYING MECHANISMS

裂殖体与蜗牛的相容性：潜在机制

• 批准号: 3444511
• 负责人: CHRISTOPHER JEFFREY BAYNE
• 金额: $ 14.03万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-09-30 至 1986-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3444511
• 关键词: cell cell interaction; cellular immunity; communicable disease transmission; host organism interaction; immunochemistry; membrane activity; microorganism immunology; protozoal antigen; schistosomiasis; tissue /cell culture

This research is aimed at providing an understanding of the mechanisms used by Schistosoma mansoni to survive and multiply in its intermediate host snail Biomphalaria glabrata. S. mansoni is a causative agent of human schistosomiasis, a serious debilitating disease in human populations in many areas of the tropics. On the premise that the compatibility of S. mansoni with B. glabrata is a highly specific phenomenon, and that the specificity is manifested as a result of events occurring at the interface of the sporocyst with host-snail hemocyte plasma membranes, we aim to provide information on the cellular-chemical basis of specificity. Experiments will be designed to yield information which will support or refute various hypotheses that bear directly on the following issues: (a) The mechanisms by which snail hemocytes recognize trematode sporocysts (b) The mechanisms by which snails acquire enhanced resistance, of a more or less specific nature, to trematode sporocysts (c) The mechanisms by which trematode sporocysts avoid destruction by hemocytes of certain snail strains (d) The mechanisms by which trematode sporocysts interfere with innate resistance of snails Methodologies will involve in vitro culture of schistosome sporocyst larvae together with immunocompetent leukocytes (hemocytes) of host snails. Both susceptible and resistant strains of snail will be used as sources of hemocytes. Sensitive labeling of potentially important molecules of host and parasite origin will permit us to evaluate their roles in mediation of compatibility.

这项研究旨在提供对所用机制的理解 曼氏血吸虫在其中间宿主中生存和繁殖 光滑双脐螺 S.曼森氏菌是人类的病原体， 血吸虫病是一种严重的使人衰弱的疾病， 许多热带地区。 在S. mansoni和B。glabrata是一种高度特异性的现象， 特异性表现为在界面处发生的事件的结果 对于带有宿主蜗牛血细胞质膜的孢子囊，我们的目标是 提供关于特异性的细胞化学基础的信息。 实验将被设计为产生将支持或 驳斥与下列问题直接相关的各种假设： (a)蜗牛血细胞识别吸虫孢子囊的机制 (b)蜗牛获得增强抵抗力的机制， 或不那么特异的性质，对吸虫孢子囊 (c)吸虫孢子囊避免被破坏的机制 某些蜗牛品系的血细胞 (d)吸虫孢子囊干扰先天免疫的机制 螺的抵抗力 方法学将涉及体外培养的寄生孢子囊幼虫 以及宿主蜗牛的免疫活性白细胞（血细胞）。 两 敏感和耐药的蜗牛菌株将被用作 血细胞 宿主潜在重要分子的灵敏标记 和寄生虫的起源将使我们能够评估它们在介导 兼容性.