CALCIUM AND PHOSPHORUS HOMEOSTASIS DURING REPRODUCTION

繁殖过程中的钙和磷稳态

• 批准号: 3446453
• 负责人: ROBERT J BROMMAGE
• 金额: $ 4.81万
• 依托单位: CHICAGO COLLEGE OF OSTEOPATHIC MEDICINE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3446453
• 关键词: 1,25 dihydroxycholecalciferol; 25 hydroxycholecalciferol; calcium disorder; calcium metabolism; dietary constituent; gastrointestinal absorption /transport; homeostasis; hormone regulation /control mechanism; lactation; phosphorus; prolactin; reproduction; secretion; skeletal system; vitamin D deficiency

The major objective of the experiments proposed in this application is to further understand the hormonal regulation of Ca and P homeostasis during reproduction. These two elements, besides being major constituents of the skeleton, play critical roles in numerous physiological processes and control of both their concentrations in body fluids and their fluxes in various tissues are essential to the normal functioning of the organism. The following experiments were designed to be performed in rats. (1) Determine if vitamin D influences prolactin secretion by comparing plasma prolactin levels in vitamin D-deficient and -replete rats at various stages of reproduction. (2) Measure renal 25-OH-D3-1-hydroxylase activity in pups during the weaning period to determine the requirement for 1,25-(OH)2D3 to stimulate intestinal Ca transport and bone mineral mobilization. (3) Measure Ca and P balances throughout lactation to determine the relative contributions of maternal diet and skeletal reserves as sources of Ca and P in milk. (4) Examine the role of vitamin D-independent mechanisms in the stimulation of intestinal Ca transport during lactation by measuring intestinal hypertrophy and Ca balances in vitamin D-deficient rats.

本申请中提出的实验的主要目标是 进一步了解激素对钙磷稳态的调节作用 繁殖。这两个元素除了是 骨骼，在许多生理过程中起着关键作用 控制它们在体液中的浓度和它们在体内的通量 各种组织对生物体的正常功能是必不可少的。 以下实验是为在大鼠身上进行的。 (1)通过比较确定维生素D是否影响催乳素的分泌 维生素D缺乏和补充大鼠不同时期血浆催乳素水平的变化 生殖的各个阶段。 (2)测定仔猪肾脏25-OH-D3-1-羟基酶活性 断奶期确定1，25-(OH)2D3刺激所需 肠道钙转运和骨矿物质动员。 (3)测定整个哺乳期的钙磷平衡，以确定 作为营养来源的母亲饮食和骨骼储备的相对贡献 牛奶中的钙和磷。 (4)研究维生素D非依赖性机制在刺激中的作用 通过测定肠道组织来研究哺乳期肠道钙转运 维生素D缺乏大鼠的肥大和钙平衡。