CALCIUM AND PHOSPHORUS HOMEOSTASIS DURING REPRODUCTION

繁殖过程中的钙和磷稳态

• 批准号: 3446274
• 负责人: ROBERT J BROMMAGE
• 金额: $ 5.15万
• 依托单位: MICHAEL REESE HOSP & MED CTR (CHICAGO)
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-01 至 1986-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3446274
• 关键词: 1,25 dihydroxycholecalciferol; 25 hydroxycholecalciferol; calcium disorder; calcium metabolism; dietary constituent; gastrointestinal absorption /transport; homeostasis; hormone regulation /control mechanism; lactation; phosphorus; prolactin; reproduction; secretion; skeletal system; vitamin D deficiency

The major objective of the experiments proposed in this application is to further understand the hormonal regulation of Ca and P homeostasis during reproduction. These two elements, besides being major constituents of the skeleton, play critical roles in numerous physiological processes and control of both their concentrations in body fluids and their fluxes in various tissues are essential to the normal functioning of the organism. The following experiments were designed to be performed in rats. (1) Determine if vitamin D influences prolactin secretion by comparing plasma prolactin levels in vitamin D-deficient and -replete rats at various stages of reproduction. (2) Measure renal 25-OH-D3-1-hydroxylase activity in pups during the weaning period to determine the requirement for 1,25-(OH)2D3 to stimulate intestinal Ca transport and bone mineral mobilization. (3) Measure Ca and P balances throughout lactation to determine the relative contributions of maternal diet and skeletal reserves as sources of Ca and P in milk. (4) Examine the role of vitamin D-independent mechanisms in the stimulation of intestinal Ca transport during lactation by measuring intestinal hypertrophy and Ca balances in vitamin D-deficient rats.

本申请中提出的实验的主要目的是 进一步了解钙和磷稳态的激素调节过程中 生殖 这两个元素，除了是主要的组成部分， 骨骼在许多生理过程中起着关键作用， 控制它们在体液中的浓度和它们在 各种组织对于生物体的正常功能是必不可少的。 以下实验设计在大鼠中进行。 (1)确定维生素D是否影响催乳素分泌，通过比较 维生素D缺乏和维生素D充足大鼠血浆催乳素水平 繁殖阶段。 (2)测量幼鼠肾脏25-OH-D3-1-羟化酶活性， 断奶期，以确定1，25-（OH）2D 3刺激的需求 肠钙转运和骨矿物质动员。 (3)测量整个哺乳期的钙和磷平衡，以确定 母体饮食和骨骼储备作为 牛奶中的钙和磷 (4)检查维生素D非依赖性机制在刺激中的作用 哺乳期肠道钙转运的研究 维生素D缺乏大鼠心肌肥厚和钙平衡。