STUDIES OF SPERM CAPACITATION AND HYPERACTIVATION

这项研究的目的是阐明 精子获能和受精的过度激活的精子活力 研究与过度激活的开始相关的分子事件。 只观察到一小部分人类精子经历了 体外过度激活，但它是基于对 一些哺乳动物物种，在受精之前过度活跃 活着。仓鼠将被用作模型系统，因为仓鼠 精子在体外可被诱导在特定的介质中高度激活 也可以观察到向受精地点移动的过程 输卵管。使用的方法是对发病情况进行对比视频分析。 在体外和体内使用标记的凝集素和 用于监测细胞表面变化的单抗 并鉴定与之相关的膜成分。 过度激活，并比较非过度激活的成功率 精子与高度激活的精子在实现部位上的差异 在输卵管内受精并穿透卵丘基质。 从仓鼠系统中学到的信息最终可能被应用于 对人类受精的理解，例如正常的精子 必须能够在正确的环境条件下高度激活 以及如何将过度活跃作为一种避孕技术来防止。

The goals of this research are to elucidate the relationship of hyperactivated sperm motility to sperm capacitation and fertilization and to examine molecular events associated with the onset of hyperactivation. Only small percentages of human spermatozoa have been observed undergoing hyperactivation in vitro, yet it is assumed on the basis of studies of several mammalian species, that hyperactivation precedes fertilization in vivo. The hamster will be used as a model system because hamster spermatozoa may be induced to hyperactivate in a defined medium in vitro and may also be observed moving towards the site of fertilization in the oviduct. The methods to be used are comparative videoanalysis of the onset of hyperactivated motility in vitro and in vivo, using labeled lectins and monoclonal antibodies to monitor cell surface changes during hyperactivation and to identify membrane components associated with hyperactivation, and comparing the success of non-hyperactivated spermatozoa with that of hyperactivated spermatozoa in achieving the site of fertilization in the oviduct and penetrating the cumulus matrix. Information learned from the hamster system could eventually be applied to the understanding of human fertilization, e.g. whether normal spermatozoa must be able to hyperactivate under the correct environmental conditions and how hyperactivation might be prevented as a contraceptive technique.