STUDIES OF SPERM CAPACITATION AND HYPERACTIVATION

本研究的目的是阐明 超活化精子活力对精子获能和受精的影响， 来检测与过度激活相关的分子事件 只有一小部分的人类精子被观察到经历了 超活化在体外，但它是假设的基础上的研究， 几种哺乳动物物种，在受精前过度激活， vivo. 仓鼠将被用作模型系统，因为仓鼠 在体外，精子可以在限定培养基中被诱导过度活化 也可以观察到向受精点移动， 输卵管 所使用的方法是比较视频分析的发病 超活化运动在体外和体内，使用标记的凝集素和 单克隆抗体监测细胞表面的变化， 超活化和识别与 超激活，并比较非超激活的成功 精子与超活化精子在达到该部位 在输卵管中受精并穿透卵丘基质。 从仓鼠系统中获得的信息最终可以应用于 对人类受精的理解，例如正常的精子 必须能够在正确的环境条件下超激活 以及作为一种避孕技术如何防止过度激活。

The goals of this research are to elucidate the relationship of hyperactivated sperm motility to sperm capacitation and fertilization and to examine molecular events associated with the onset of hyperactivation. Only small percentages of human spermatozoa have been observed undergoing hyperactivation in vitro, yet it is assumed on the basis of studies of several mammalian species, that hyperactivation precedes fertilization in vivo. The hamster will be used as a model system because hamster spermatozoa may be induced to hyperactivate in a defined medium in vitro and may also be observed moving towards the site of fertilization in the oviduct. The methods to be used are comparative videoanalysis of the onset of hyperactivated motility in vitro and in vivo, using labeled lectins and monoclonal antibodies to monitor cell surface changes during hyperactivation and to identify membrane components associated with hyperactivation, and comparing the success of non-hyperactivated spermatozoa with that of hyperactivated spermatozoa in achieving the site of fertilization in the oviduct and penetrating the cumulus matrix. Information learned from the hamster system could eventually be applied to the understanding of human fertilization, e.g. whether normal spermatozoa must be able to hyperactivate under the correct environmental conditions and how hyperactivation might be prevented as a contraceptive technique.