MECHANISMS OF INDUCED TOLERANCE TO UV RADIATION IN SKIN

皮肤对紫外线辐射的诱导耐受机制

• 批准号: 3456670
• 负责人: RICHARD W GANGE
• 金额: $ 7.74万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-20 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3456670
• 关键词: DNA; erythema; human subject; melanins; melanocyte; nonvisual photosensitivity; photoprotection; pigmentation; radiation protection; skin; sunburn; ultraviolet radiation

We will use quantitative techniques to measure the protective responses of normal skin to UVR as a function of heritable melanogenic capability and as a function of ultraviolet waveband. We will examine both melanin and non-melanin mechanisms. For the first time we will correlate structural and optical measurements in vitro with protection against erythema and against specific markers of epidermal and dermal damage. Responses will be compared in individuals of different genetic tanning capability and in response to different wavebands (UVA and UVB). UV-induced structural changes and alterations in optical transmissions by isolated epidermis and stratum corneum samples will be measured and correlated with the development of tolerance to insult by UVR. Tolerance will be assessed by measuring the degree of erythema, structural damage to dermal blood vessels, and damage to DNA in the epidermis resulting from further UV exposure. These studies will determine the relative importance of different protective mechanisms in subjects of different heritable pigmentation capabilities. We will determine whether Caucasians are heterogeneous with respect to their ability to develop tolerance to UVR by non-melanin mechanisms and, if so, whether differences correlate with or are dependent of tanning ability. The effects of UVA exposure upon melanocyte populations will be examined in different skin types, and the functional contribution of longwave induced changes to induced photoprotection will be examined.

我们将使用定量技术来衡量保护反应 正常皮肤对 UVR 的影响是遗传性黑色素生成能力的函数， 紫外线波段的函数。 我们将检查黑色素和 非黑色素机制。 我们第一次将结构关联起来 和体外光学测量，可防止红斑和 针对表皮和真皮损伤的特定标记。 回应将是 在不同基因晒黑能力的个体中进行比较 对不同波段（UVA 和 UVB）的响应。 紫外线诱导结构 孤立的表皮和光传输的变化和改变 角质层样本将被测量并与 发展对 UVR 损伤的耐受性。 耐受性将通过以下方式评估 测量红斑程度、真皮血液结构损伤 血管，以及进一步紫外线对表皮 DNA 的损伤 接触。 这些研究将确定以下方面的相对重要性： 不同遗传基因的受试者有不同的保护机制 色素沉着能力。 我们将确定白人是否是 就其发展对 UVR 耐受性的能力而言，异质性 非黑色素机制，如果是，差异是否相关或 取决于晒黑能力。 UVA照射对人体的影响 将在不同皮肤类型中检查黑色素细胞群，并且 长波诱导变化对诱导的功能贡献 将检查光保护。